The EasySep™ Human EpCAM Positive Selection Kit II is designed to isolate EpCAM+ cells from fresh or previously frozen human mammary epithelial cell preparations by immunomagnetic positive selection. Desired cells are targeted with antibody complexes recognizing EpCAM and dextran-coated magnetic particles. The cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The EpCAM+ fraction contains luminal cells, luminal-restricted, and bipotential progenitor cells.
This product replaces the EasySep™ Human EpCAM Positive Selection Kit (Catalog #18356).
This product replaces the EasySep™ Human EpCAM Positive Selection Kit (Catalog #18356).
Data Figures
Starting with peripheral blood mononuclear cells (PBMCs) seeded with MCF-7 cells (breast cancer cell line) at a starting frequency of 10.8 - 50.0%, the EpCAM+ cell content (epithelial cell+CD45-)of the isolated fraction is typically 96.2 ± 3% (mean ± SD using the purple EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 10.8% and 95.0%, respectively.
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
EasySep™ Human EpCAM Positive Selection Kit II
Catalog #
17846
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
RoboSep™ Human EpCAM Positive Selection Kit II
Catalog #
17846RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Human EpCAM Positive Selection Kit II
Catalog #
17846
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Human EpCAM Positive Selection Kit II
Catalog #
17846
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
RoboSep™ Human EpCAM Positive Selection Kit II
Catalog #
17846RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
RoboSep™ Human EpCAM Positive Selection Kit II
Catalog #
17846RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
RoboSep™ Human EpCAM Positive Selection Kit II
Catalog #
17846RF
Lot #
All
Language
English
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
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Workflow Stages
Resources and Publications
Educational Materials (5)
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