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EasySep™人EpCAM正选试剂盒II

免疫磁珠正选试剂盒
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产品号 #（选择产品）

产品号 #17846_C

免疫磁珠正选试剂盒

产品优势

  • 操作简单、快速
  • 纯度高达99%
  • 无需分离柱

产品组分包括

  • EasySep™人EpCAM 正选试剂盒II（产品号 ＃17846）
    • EasySep™人EpCAM 正选抗体混合物，1 mL
    • EasySep™ Dextran RapidSpheres™ 50100 磁珠，1 mL
  • RoboSep™ 人EpCAM 正选试剂盒II（含过滤吸头）（产品号＃17846RF）
    • EasySep™人EpCAM 正选抗体混合物，1 mL
    • EasySep™ Dextran RapidSpheres™ 50100 磁珠，1 mL
    • RoboSep™ 缓冲液（产品号 #20104）
    • RoboSep™过滤吸头（产品号 #20125）
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总览
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产品说明书及文档
应用领域
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总览

EasySep™人EpCAM 正选试剂盒II旨在通过免疫磁珠正选，从新鲜或冻存的人类乳腺上皮细胞样本中分离EpCAM+ 细胞。目的细胞被识别EpCAM+的抗体复合物和磁珠标记。分选抗体混合物中还含有人Fc受体抗体，可最大程度地减少非特异性结合。通过EasySep™磁极分离被标记的细胞，无需使用分离柱。目的细胞被保留在试管中，而非目的细胞被倾倒出。EpCAM+部分包含管腔细胞（luminal cells）、管腔限制性细胞（luminal-restricted）及双潜能祖细胞（bipotential progenitor cells）。

本产品可替代 EasySep™ 人EpCAM正选试剂盒（产品号 #18356）。

磁体兼容性
• EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • RoboSep™-S (Catalog #21000)
 
亚型
细胞分选试剂盒
 
细胞类型
乳腺细胞
 
种属
人
 
样本来源
其它细胞系，Primary
 
筛选方法
Positive
 
应用
细胞分选
 
品牌
EasySep，RoboSep
 
研究领域
癌症，上皮细胞研究
 

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Data Figures

Starting with peripheral blood mononuclear cells (PBMCs) seeded with MCF-7 cells (breast cancer cell line) at a starting frequency of 10.8 - 50.0%, the EpCAM+ cell content (epithelial cell+CD45-)of the isolated fraction is typically 96.2 ± 3% (mean ± SD using the purple EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 10.8% and 95.0%, respectively.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
EasySep™ Human EpCAM Positive Selection Kit II
Catalog #
17846
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
RoboSep™ Human EpCAM Positive Selection Kit II
Catalog #
17846RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Human EpCAM Positive Selection Kit II
Catalog #
17846
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Human EpCAM Positive Selection Kit II
Catalog #
17846
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
RoboSep™ Human EpCAM Positive Selection Kit II
Catalog #
17846RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
RoboSep™ Human EpCAM Positive Selection Kit II
Catalog #
17846RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
RoboSep™ Human EpCAM Positive Selection Kit II
Catalog #
17846RF
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

更多信息

更多信息
Species Human
Magnet Compatibility • EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • RoboSep™-S (Catalog #21000)
Sample Source Other, Primary
Selection Method Positive
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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