Figure 1. Typical EasySep™ HLA Chimerism Buffy Coat CD56 Positive Selection Profile
Starting with freshly prepared buffy coat, the CD56+ cell content of the isolated fraction typically ranges from 95.8 - 99.5% (gated on CD45+ using “The Big Easy” EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 8.5% and 98.4%, respectively. NOTE: RBCs were removed from the start sample by lysis prior to flow cytometry.
Figure 2. FACS Data for Anti-Human CD56 Antibody, Clone HCD56, APC-Conjugated
Flow cytometry analysis of human buffy coat nucleated cells processed with the EasySep™ HLA Buffy Coat CD56 Positive Selection Kit (Catalog #17875) and labeled with Anti-Human CD56 Antibody, Clone HCD56, APC (Catalog #60021AZ). Histograms show labeling of buffy coat nucleated cells (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa APC isotype control antibody is shown (solid line histogram).
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