EasySep™ Direct人嗜碱性粒细胞分选试剂盒

全血免疫磁珠负选试剂盒

产品号 #19667

直接从全血中免疫磁珠分选嗜碱性粒细胞

产品优势

  • 高效去除红细胞,无需密度梯度离心、沉降或裂解
  • 分选获得的细胞纯度高达98%
  • 操作简单、快捷,且无需分离柱
  • 分选得到的细胞不带标记

产品组分包括

  • EasySep™ Direct人嗜碱性粒细胞分选试剂盒 (产品号 #19667)
    • EasySep™ Direct人嗜碱性粒细胞分选抗体混合物, 2 x 2.5 mL
    • EasySep™ Direct RapidSpheres™磁珠, 4 x 2.5 mL

概述

EasySep™ Direct人嗜碱性性粒细胞分选试剂盒通过免疫磁珠负选直接从人全血中分离功能性的、高纯度嗜碱性粒细胞。无需裂解、密度梯度离心或其他处理步骤,分离得到的细胞可立即用于流式细胞术、功能检测、培养或其他下游应用。

MAGNET COMPATIBILITY
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• Easy 50 EasySep™ Magnet (Catalog #18002)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
 
SUBTYPE
Cell Isolation Kits
 
CELL TYPE
T Cells, T Cells, CD8+
 
SPECIES
Human
 
SAMPLE SOURCE
Whole Blood
 
SELECTION METHOD
Negative
 
APPLICATION
Cell Isolation
 
BRAND
EasySep
 
AREA OF INTEREST
Immunology

实验数据

Figure 1. Typical EasySep™ Direct Human Basophil Isolation Profile

Starting with human whole blood from normal healthy donors, the typical basophil (CD123+IgE+) content of the non-lysed final isolated fraction is 97.3 ± 1.1% (gated on CD45). In the above example, the basophil content of the lysed whole blood start sample and the non-lysed final isolated fraction is 0.73% and 96.0% (gated on CD45), respectively, or 0.68% and 89.8% (not gated on CD45), respectively.

产品说明书及文档

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
19667
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Catalog #
19667
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Catalog #
19667
Lot #
All
Language
English

应用领域

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

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