Use Deoxyribonuclease I (DNase I), Animal Component-Free (ACF) for routine tissue dissociation, to minimize cell clumping, and eliminate DNA contaminants from dissociation medium. Obtained from cultures free of animal-derived materials and purified by chromatography, this endonuclease consists of a single glycosylated polypeptide chain with two disulfide bonds. It preferentially cleaves phosphodiester linkages adjacent to pyrimidine nucleotides in both single- and double-stranded DNA, yielding polynucleotides with 5’-phosphate and 3’-hydroxyl groups (Bernardi et al.). DNase I has been used for DNA digestion in human cells and tissues such as microglia (Klegeris & McGeer), cartilage (Dunham & Koch), colon (Fukushima & Fiocchi), epithelium (Fukushima & Fiocchi), liver (Vatakis et al.), lung (Fujino et al.), neural cells (Fuja et al.), and stem cells (Kusuma et al.).
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Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
DNase I, ACF
Catalog #
07473, 100-0273, 07474
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
DNase I, ACF
Catalog #
07473, 100-0273, 07474
Lot #
All
Language
English
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages
Workflow Stages for B Cell Research
Workflow Stages for Dendritic Cell Research
Workflow Stages for Granulocyte Research
Workflow Stages for Total Lymphocyte Research
Workflow Stages for Mammary Epithelial Cell Research
Workflow Stages for Monocyte Research
Workflow Stages for Myeloid Cell Research
Workflow Stages for Natural Killer Cell Research
Workflow Stages for Prostate Epithelial Cell Research
Workflow Stages for T Cell Research
Workflow Stages for HLA Analysis
Workflow Stages for Chimerism Analysis
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