Save time and your precious clones during cell line development by increasing cloning efficiency and promoting robust growth of Chinese hamster ovary (CHO) cells with ClonaCell™-CHO AOF Supplement in your base medium.
Formulated without phenol red, this supplement offers precipitate-free culture for improved imaging clarity and visualization of clonality, compared to ClonaCell™-CHO ACF Supplement (Catalog #03820). This animal origin-free (AOF) supplement is formulated with no animal-derived raw materials to the secondary level of manufacturing. It contains recombinant proteins and chemically defined components and is free of serum, hydrolysates, L-glutamine, or selection agents.
Figure 1. Cloning Efficiencies for Subcloned CHO-S and CHO-K1 Cells
CHO-S (A) and CHO-K1 (B) cells were subcloned by limiting dilution in DMEM + 10% FBS, or ClonaCell™-CHO CD Liquid Medium containing either ClonaCell™-CHO ACF Supplement or ClonaCell™-CHO AOF Supplement; all conditions contained 6 - 8 mM L-glutamine. Individual wells of 96-well plates were seeded with an average of 0.5 or 1 cell per well in 200 μL culture medium. After incubation for 14 days (37°C, 5% CO2) the plates were examined under a microscope and assessed for growth, with wells containing >100 cells considered positive for outgrowth. The cloning efficiency was estimated by Poisson statistics using the ELDA method described by Hu & Smith (2009). Data are shown as mean ± 1 SD for n = 4 independent experiments.
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