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ClonaCell™-CHO AOF 添加物

无动物来源、无血清、无L-谷氨酰胺和无酚红的40X培养添加物，用于CHO细胞开发过程中的克隆
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产品号 #100-0382_C

无动物来源、无血清、无L-谷氨酰胺和无酚红的40X培养添加物，用于CHO细胞开发过程中的克隆

产品优势

  • 显著提高CHO细胞在无蛋白液体或半固体培养基中的单细胞克隆效率；
  • 兼容多种市售的液体或半固体无蛋白培养基；
  • 支持用户维护现有的上下游细胞培养流程。
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总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
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总览

通过在基础培养基中使用ClonaCell™-CHO AOF添加物，提高克隆效率，促进中国仓鼠卵巢（CHO）细胞的强劲生长，从而节省时间和保护宝贵的克隆细胞。配方不含酚红，与ClonaCell™-CHO ACF 添加物（产品号：#03820）相比，该添加物提供无沉淀培养，可提高成像清晰度和克隆性可视化。

这款无动物来源（AOF）添加物在二级生产过程中不含动物来源的原材料。它含有重组蛋白和化学成分明确，不含血清、水解物、L -谷氨酰胺或选择剂。此40X添加物可与多种适用于CHO 细胞培养的基础培养基一起使用，包括无蛋白ClonaCell™-CHO CD液体培养基（产品号：#03817）或半固体ClonaCell™-CHO CD培养基（产品号：#03815）。在ClonaCell™品牌页面中探索高效细胞克隆和细胞系生成的液体和半固体培养基。了解更多关于我们如何定义我们的培养基和添加物，包括AOF培养基。

亚型
添加剂
 
细胞类型
CHO细胞
 
种属
其它细胞系
 
应用
细胞培养
 
品牌
ClonaCell
 
研究领域
抗体​制备，细胞系制备，药物发现和毒理检测
 
制剂类别
Animal Origin-Free，无血清
 

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Data Figures

Comparison of the cloning efficiency (%) of CHO-S and CHO-K1 cells subcloned in DMEM + 10% FBS, or ClonaCell™-CHO CD Liquid Medium containing either ClonaCell™-CHO ACF Supplement or ClonaCell™-CHO AOF Supplement.

Figure 1. Cloning Efficiencies for Subcloned CHO-S and CHO-K1 Cells

CHO-S (A) and CHO-K1 (B) cells were subcloned by limiting dilution in DMEM + 10% FBS, or ClonaCell™-CHO CD Liquid Medium containing either ClonaCell™-CHO ACF Supplement or ClonaCell™-CHO AOF Supplement; all conditions contained 6 - 8 mM L-glutamine. Individual wells of 96-well plates were seeded with an average of 0.5 or 1 cell per well in 200 μL culture medium. After incubation for 14 days (37°C, 5% CO2) the plates were examined under a microscope and assessed for growth, with wells containing >100 cells considered positive for outgrowth. The cloning efficiency was estimated by Poisson statistics using the ELDA method described by Hu & Smith (2009). Data are shown as mean ± 1 SD for n = 4 independent experiments.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
ClonaCell™-CHO AOF Supplement
Catalog #
100-0382
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
ClonaCell™-CHO AOF Supplement
Catalog #
100-0382
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

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Species Other
Formulation Category Animal Origin-Free, Serum-Free
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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