产品号 #100-0784_C
cGMP，人T细胞激活扩增试剂
总览
实现人T细胞的稳定激活和扩增，用于临床应用—无需使用磁珠，饲养细胞或抗原。
本品温和的激活刺激方式保证了活化T细胞的高活性，可以搭配ImmunoCult™- XF培养基实现更优越的扩增效果，该培养基是一种根据相关cGMP法规生产的高性能T细胞扩增培养基。ImmunoCult™人CD3/CD28 T细胞激活剂由可溶性抗体复合物组成，可与细胞表面配体CD3和CD28结合并交联，从而为T细胞激活和培养提供所需的初级和共刺激信号。
ImmunoCult™人CD3/CD28 T细胞激活剂是专为细胞治疗临床研究应用而设计的，符合USP<1043> 和/或PH. EUR.5.2.12.中规定的框架，可作为辅助材料（AM）使用。STEMCELL可与您合作，根据已获批的临床试验新药（IND）申请，生物制品许可申请（BLA）或临床试验申请（CTA），将该试剂认证为辅助材料。点击此处，了解更多关于我们如何支持您的法规需求的信息。
包含
• Anti-human CD3 monospecific antibody complex
• Anti-human CD28 monospecific antibody complex
• Phosphate buffered-saline (PBS)，containing 0.02% TWEEN® 20
亚型
添加剂
细胞类型
T 细胞，T 细胞，CD4+，T 细胞，CD8+
种属
人
应用
激活，细胞培养，扩增
品牌
ImmunoCult
研究领域
癌症，免疫，细胞治疗开发
Data Figures
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Resources and Publications
Educational Materials (11)
Publications (2)
Th17 cell-derived miR-155-5p modulates interleukin-17 and suppressor of cytokines signaling 1 expression during the progression of systemic sclerosis. Journal of clinical laboratory analysis 2022 jun
Abstract
BACKGROUND miR-155-5p is associated with autoimmune diseases. T helper 17 (Th17) cells, interleukin (IL)-17, and suppressor of cytokines signaling 1 (SOCS1) have important roles in the pathogenesis of systemic sclerosis (SSc). The purpose of this study was to explore the role of miR-155-5p in the regulation of IL-17 and SOCS1 expression in Th17 cells and the subsequent effect on SSc disease progression. METHODS Th17 cells were isolated from peripheral blood mononuclear cells of SSc patients and healthy controls (HCs). RT-qPCR and western blotting were used to examine the expression patterns of miR-155-5p, IL-17, and SOCS1. Luciferase reporter assays were performed to confirm SOCS1 as a target of miR-155-5p. RNA pull-down assays were performed to detect the interaction of IL-17 and SOCS1 with miR-155-5p. In situ hybridization was performed to analyze the co-expression pattern of miR-155-5p and IL17A in Th17 cells. RESULTS The levels of Th17 cell-derived miR-155-5p were significantly up-regulated in SSc patients compared with HCs, and its levels were negatively correlated with SOCS1 levels. Meanwhile, miR-155-5p positively regulated IL-17 expression levels in Th17 cells isolated from SSc patients as the disease progressed. Using pmirGLO vectors, SOCS1 was confirmed as a target of miR-155-5p. The binding status of IL-17 and SOCS1 to miR-155-5p was related to SSc progression. An increase in the co-localization of miR-155-5p and IL-17 was associated with greater SSc progression. CONCLUSIONS IL-17 and SOCS1 expression modulated by Th17 cell-derived miR-155-5p are critical for SSc progression, which may provide novel insights into the pathogenesis of SSc.
Stress hormone signalling inhibits Th1 polarization in a CD4 T-cell-intrinsic manner via mTORC1 and the circadian gene PER1. Immunology 2022 apr
Abstract
Stress hormones are believed to skew the CD4 T-cell differentiation towards a Th2 response via a T-cell-extrinsic mechanism. Using isolated primary human na{\{i}}ve and memory CD4 T cells here we show that both adrenergic- and glucocorticoid-mediated stress signalling pathways play a CD4 na{\"{i}}ve T-cell-intrinsic role in regulating the Th1/Th2 differentiation balance. Both stress hormones reduced the Th1 programme and cytokine production by inhibiting mTORC1 signalling via two parallel mechanisms. Stress hormone signalling inhibited mTORC1 in na{\"{i}}ve CD4 T cells (1) by affecting the PI3K/AKT pathway and (2) by regulating the expression of the circadian rhythm gene period circadian regulator 1 (PER1). Both stress hormones induced the expression of PER1 which inhibited mTORC1 signalling thus reducing Th1 differentiation. This previously unrecognized cell-autonomous mechanism connects stress hormone signalling with CD4 T-cell differentiation via mTORC1 and a specific circadian clock gene namely PER1."
更多信息
| Species | Human |
|---|---|
| Contains | • Anti-human CD3 monospecific antibody complex • Anti-human CD28 monospecific antibody complex • Phosphate buffered-saline (PBS), containing 0.02% TWEEN® 20 |
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ImmunoCult™人CD3/CD28/CD2 T细胞激活剂cGMP，人T细胞激活和扩增试剂
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ImmunoCult™ XF培养基cGMP，无血清和无异种成分培养基，用于人T细胞扩增
THIS PRODUCT IS MANUFACTURED AND TESTED FOLLOWING RELEVANT CGMPs UNDER A CERTIFIED QUALITY MANAGEMENT SYSTEM. PRODUCT IS FOR INVESTIGATIONAL OR RESEARCH USE. NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
