产品号 #75003_C
Cell proliferation and tracking dye
概述
CFDA-SE (5(6)-carboxyfluorescein diacetate succinimidyl ester) is a stable, cell-permeable diacetate precursor to CFSE. Upon diffusion into the cell, intracellular esterases cleave the acetate group to generate CFSE, which interacts with cellular amines via its succinimidyl groups to generate a highly fluorescent green dye that is impermeant to the cell membrane. CFDA-SE is frequently used in cell proliferation assays, as it is partitioned approximately equally between the progeny so that cell division can be followed as a successive halving of the fluorescence intensity through multiple generational divisions. CFDA-SE is also used for motility assays and in vivo cell tracking experiments. CFDA-SE-labeled cells can be detected with any instrument/filter set compatible with fluorescein detection.
Alternative Names
5(6)-Carboxyfluorescein succinimidyl ester; 6-{[(2,5-Dioxo-1-pyrrolidinyl)oxy]carbonyl}-3-oxo-3H-spiro[2- benzofuran-1,9'-xanthene]-3',6'-diyl diacetate; carboxyfluorescein diacetate succinimidyl ester; CFSE
Cell Type
Other
Species
Human, Mouse, Non-Human Primate, Other, Rat
Application
Flow Cytometry, Immunocytochemistry, Immunofluorescence
Area of Interest
Immunology, Neuroscience, Stem Cell Biology
CAS Number
150347-59-4
Chemical Formula
C₂₉H₁₉NO₁₁
Molecular Weight
557.5 g/mol
Data Figures
(A) Flow cytometry analysis of Sp2/0 mouse myeloma cells labeled with CFDA-SE and analyzed by flow cytometry after being cultured for 0, 24, and 48 hours (filled histograms). Solid line histogram (control) shows unlabeled cells analyzed after 48 hours of cell culture. (B) Chemical structure of CFDA-SE.
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet Product Name
CFDA-SE Catalog #
75003 Lot #
All Language
English Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages
Workflow Stages for B Cell Research
Workflow Stages for Dendritic Cell Research
Workflow Stages for Granulocyte Research
Workflow Stages for Total Lymphocyte Research
Workflow Stages for Mammary Epithelial Cell Research
Workflow Stages for Mesenchymal Stem and Progenitor Cell Research
Workflow Stages for Monocyte Research
Workflow Stages for Myeloid Cell Research
Workflow Stages for Neural Stem and Progenitor Cell Research
Workflow Stages for Natural Killer Cell Research
Workflow Stages for T Cell Research
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