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翻译:SepMate™-50 (RUO)

密度梯度离心管仅供研究使用
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产品号 #（选择产品）

产品号 #86450_C

密度梯度离心管仅供研究使用

产品优势

  • 无需在密度梯度介质上仔细分层血液（例如Ficoll-Paque™，lymphooprep™）
  • 减少总离心机时间到10分钟与制动新鲜样品
  • 通过简单地倒出上清，可以快速和容易地收获分离的单个核细胞
  • 能否与RosetteSep™浓缩鸡尾酒结合，在25分钟内分离特定细胞类型

产品组分包括

  • SepMate™-50 (RUO)， 100支（目录#86450）
    • 分配器盒包含4袋，25支/袋
  • SepMate™-50 (RUO)， 500管（目录#86460）
    • 分配器盒包含4袋，25管/袋（目录#86450）x 5
main product photo
Try SepMate™-50 tubes for your density gradient centrifugation. Request a Sample
Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.
  1. Lymphoprep™
    Lymphoprep™

    Density gradient medium for the isolation of mononuclear cells

  2. Dulbecco's Phosphate Buffered Saline with 2% Fetal Bovine Serum
    Dulbecco's Phosphate Buffered Saline with 2% ...

    Cell culture buffer

总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
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概述

SepMate™是一种便于通过密度梯度离心分离pbmc或特定细胞类型的管。SepMate™管包含一个插入物，在密度梯度介质和血液之间提供屏障。SepMate™消除了将血液（或骨髓或脐带血）仔细分层到密度梯度介质上的需要，并且可以通过简单的倒液快速轻松地收集分离的单个核细胞。SepMate™还与RosetteSep™浓缩鸡尾酒兼容，可在不到30分钟内分离特定细胞类型。

SepMate™-50专为处理4至17 mL样品而设计。

SepMate™-50 (RUO)（目录#86450和#86460）在cGMP下生产，仅供研究使用。

浏览我们的常见问题（FAQs）在SepMate™。

Contains

Polypropylene tube containing an insert
 
Subtype
Centrifugation Tubes
 
Cell Type
B Cells, Dendritic Cells, Monocytes, Mononuclear Cells, NK Cells, T Cells, T Cells, CD4+, T Cells, CD8+, T Cells, Other Subsets, T Cells, Regulatory
 
Species
Human
 
Sample Source
Bone Marrow, Cord Blood, Whole Blood
 
Selection Method
Negative
 
Application
Cell Isolation
 
Brand
SepMate
 
Area of Interest
Chimerism, HLA, Immunology
 
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Data Figures

PBMC recovery from fresh whole blood using SepMate™-50 versus standard density gradient centrifugation. Graph also shows PBMC recovery from a 48 hour-old sample using SepMate™. n in each group = 7

Figure 1. Recovery of mononuclear cells (MNCs) from peripheral blood using SepMate™-50 versus standard density gradient centrifiguation. Recovery of MNCs from fresh and 48-hour post blood draw enriched by density gradient centrifugation with SepMate™ (purple) or without (grey). There was no significant difference in the recovery of MNCS with and without SepMate™.

PBMC recovery from fresh whole blood using SepMate™-50 versus standard density gradient centrifugation. Graph also shows PBMC recovery from a 48 hour-old sample using SepMate™. n in each group = 7

Figure 2. Human CD4+ T Cell Isolation using SepMate™-50 and RosetteSep™ Human CD4+ T Cell Enrichment Cocktail

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
SepMate™-50 (RUO)
Catalog #
86450, 86460
Lot #
All
Language
English

Resources and Publications

Educational Materials (11)

Lymphoprep™ Density Gradient Medium for Mononuclear Cell Isolation
Brochure
Lymphoprep™ Density Gradient Medium for Mononuclear Cell Isolation
SepMate™ Hassle-Free PBMCs in Just 15 Minutes
Brochure
SepMate™ Hassle-Free PBMCs in Just 15 Minutes
How to Isolate Mononuclear Cells from Blood in 15 Minutes with SepMate™
Protocol
How to Isolate Mononuclear Cells from Blood in 15 Minutes with SepMate™
Cell Isolation in One Spin Using SepMate™ Tubes and RosetteSep™ Cell Separation Reagents
1:02
Video
Cell Isolation in One Spin Using SepMate™ Tubes and RosetteSep™ Cell Separation Reagents
How SepMate™ PBMC Isolation Tubes Work: From Whole Blood to PBMCs in 15 Minutes
2:28
Video
How SepMate™ PBMC Isolation Tubes Work: From Whole Blood to PBMCs in 15 Minutes
How to Speed up Your PBMC Isolations with SepMate™
18:36
Video
How to Speed up Your PBMC Isolations with SepMate™
How to Isolate PBMCs from Whole Blood Using the SepMate™ PBMC Isolation Tubes: 15-Minute Protocol
1:46
Video
How to Isolate PBMCs from Whole Blood Using the SepMate™ PBMC Isolation Tubes: 15-Minute Protocol
Fast and Easy Hematopoietic Progenitor Cell Enrichment With SepMate™
Scientific Poster
Fast and Easy Hematopoietic Progenitor Cell Enrichment With SepMate™
A Specialized Tube to Make Rosettesep™ Enrichment of Specific Cell Subsets Faster and Easier
Scientific Poster
A Specialized Tube to Make Rosettesep™ Enrichment of Specific Cell Subsets Faster and Easier
A Specialized Tube to Make Enrichment of Specific Cell Subsets Faster and Easier
Scientific Poster
A Specialized Tube to Make Enrichment of Specific Cell Subsets Faster and Easier
A Specialized Tube for Rapid PBMC Preparation, Pre-Enrichment, or Isolation of Specific Cell Populations
Scientific Poster
A Specialized Tube for Rapid PBMC Preparation, Pre-Enrichment, or Isolation of Specific Cell Populat...

Publications (27)

Knee loading repairs osteoporotic osteoarthritis by relieving abnormal remodeling of subchondral bone via Wnt/$\beta$-catenin signaling. W. Zheng et al. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2020 jan

Abstract

Osteoporotic osteoarthritis (OPOA) is a common bone disease mostly in the elderly, but the relationship between Osteoporotic (OP) and osteoarthritis (OA) is complex. It has been shown that knee loading can mitigate OA symptoms. However, its effects on OPOA remain unclear. In this study, we characterized pathological linkage of OP to OA, and evaluated the effect of knee loading on OPOA. We employed two mouse models (OA and OPOA), and conducted histology, cytology, and molecular analyses. In the OA and OPOA groups, articular cartilage was degenerated and Osteoarthritis Research Society International score was increased. Subchondral bone underwent abnormal remodeling, the differentiation of bone marrow mesenchymal stem cells (BMSCs) to osteoblasts and chondrocytes was reduced, and migration and adhesion of pre-osteoclasts were enhanced. Compared to the OA group, the pathological changes of OA in the OPOA group were considerably aggravated. After knee loading, however, cartilage degradation was effectively prevented, and the abnormal remodeling of subchondral bone was significantly inhibited. The differentiation of BMSCs was also improved, and the expression of Wnt/$\beta$-catenin was elevated. Collectively, this study demonstrates that osteoporosis aggravates OA symptoms. Knee loading restores OPOA by regulating subchondral bone remodeling, and may provide an effective method for repairing OPOA.
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High Dimensional Mass Cytometry Analysis Reveals Characteristics of the Immunosuppressive Microenvironment in Diffuse Astrocytomas. W. Fu et al. Frontiers in oncology 2020

Abstract

The tumor immune microenvironment (TIME) plays a pivotal role in tumor development, progression, and prognosis. However, the characteristics of the TIME in diffuse astrocytoma (DA) are still unclear. Leveraging mass cytometry with a panel of 33 markers, we analyzed the infiltrating immune cells from 10 DA and 4 oligodendroglioma (OG) tissues and provided a single cell-resolution landscape of the intricate immune microenvironment. Our study profiled the composition of the TIME in DA and confirmed the presence of immune cells, such as glioma-associated microglia/macrophages (GAMs), CD8+ T cells, CD4+ T cells, regulatory T cells (Tregs), and natural killer cells. Increased percentages of PD-1+ CD8+ T cells, TIM-3+ CD4+ T cell subpopulations, Tregs and pro-tumor phenotype GAMs substantially contribute to the local immunosuppressive microenvironment in DA. DAs and OGs share similar compositions in terms of immune cells, while GAMs in DA exhibit more inhibitory characteristics than those in OG.
View publication
Identification of anti-CD16a single domain antibodies and their application in bispecific antibodies. Y. Zhao et al. Cancer biology {\&} therapy 2019 sep

Abstract

CD16a (Fc$\gamma$RIIIa) mediates the antibody dependent cellular cytotoxicity (ADCC) and is important for anti-tumor activities of many therapeutic antibodies. Bispecific antibody targeting natural killer (NK) cells has been studied for cancer therapy. In this work, anti-CD16a single-domain antibodies were identified from hCD16a immunized camel. Bispecific antibodies are then constructed by fusing these single domain antibodies with an anti-CEA single domain antibody. These bispecific antibodies can recruite NK cells to kill CEA-positive tumor cells, and inhibit tumor growth in vivo, suggesting that these anti-CD16a single domain antibodies are powerful tools to engaging NK cells for cancer therapy.
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Species Human
Contains Polypropylene tube containing an insert
Sample Source Bone Marrow, Cord Blood, Whole Blood
Selection Method Negative
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