产品号 #72032_C
WNT 通路激活剂；抑制 GSK3
总览
BIO 是一种靛玉红化合物，可透过细胞，是 GSK3α 和 GSK3β 的选择性强效可逆抑制剂（IC₅₀ = 5 nM），并可作为 WNT 激活剂发挥作用。
重编程
·增强小鼠成纤维细胞、神经干细胞和胸腺细胞重编程为诱导多能干 (iPS) 细胞（Lluis et al.）。
维持和自我更新
·在没有 LIF 的情况下维持小鼠胚胎干细胞 (ES) 的自我更新（Sato et al.）。
·通过 SMAD 2/3 依赖机制维持人 ES 细胞在非培养基中的自我更新（James et al., Sato et al.）。
·诱导新生和成年大鼠心肌细胞增殖（Tseng et al.）。
分化
·促进人 ES 细胞和 iPS 细胞向心肌细胞分化（Lian et al.）。
细胞类型
心肌细胞，PSC衍生，多能干细胞
种属
人，小鼠，非人灵长类，其它细胞系，大鼠
应用
分化，扩增，培养，重编程
研究领域
干细胞生物学
CAS 编号
667463-62-9
化学式
C₁₆H₁₀BrN₃O₂
纯度
≥98%
通路
WNT
靶点
GSK3
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Resources and Publications
Educational Materials (3)
Publications (2)
Robust cardiomyocyte differentiation from human pluripotent stem cells via temporal modulation of canonical Wnt signaling. Proceedings of the National Academy of Sciences of the United States of America 2012 JUL
Abstract
Human pluripotent stem cells (hPSCs) offer the potential to generate large numbers of functional cardiomyocytes from clonal and patient-specific cell sources. Here we show that temporal modulation of Wnt signaling is both essential and sufficient for efficient cardiac induction in hPSCs under defined, growth factor-free conditions. shRNA knockdown of β-catenin during the initial stage of hPSC differentiation fully blocked cardiomyocyte specification, whereas glycogen synthase kinase 3 inhibition at this point enhanced cardiomyocyte generation. Furthermore, sequential treatment of hPSCs with glycogen synthase kinase 3 inhibitors followed by inducible expression of β-catenin shRNA or chemical inhibitors of Wnt signaling produced a high yield of virtually (up to 98%) pure functional human cardiomyocytes from multiple hPSC lines. The robust ability to generate functional cardiomyocytes under defined, growth factor-free conditions solely by genetic or chemically mediated manipulation of a single developmental pathway should facilitate scalable production of cardiac cells suitable for research and regenerative applications.
GSK-3-selective inhibitors derived from Tyrian purple indirubins. Chemistry & biology 2003 DEC
Abstract
Gastropod mollusks have been used for over 2500 years to produce the Tyrian purple" dye made famous by the Phoenicians. This dye is constituted of mixed bromine-substituted indigo and indirubin isomers. Among these�
更多信息
| Species | Human, Mouse, Non-Human Primate, Other, Rat |
|---|---|
| Cas Number | 667463-62-9 |
| Chemical Formula | C₁₆H₁₀BrN₃O₂ |
| Purity | ≥ 98% |
| Target | GSK3 |
| Pathway | WNT |
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