产品号 #60130_C
兔多克隆IgG抗体,抗小鼠Ym1
Ym1是一种~45 kDa的分泌蛋白,由激活的小鼠腹腔巨噬细胞在寄生虫感染引起的炎症反应中合成。Ym1是中性粒细胞中的颗粒蛋白,已从mev/mev和SHIP(含SH2的肌醇5′磷酸酶)敲除小鼠支气管肺泡灌洗液中形成的嗜酸性晶体中被纯化出来。在小鼠中形成的嗜酸性晶体被认为在组织学上与在人体中发现的Charcot-Leyden晶体相同。Ym1与T淋巴细胞来源的嗜酸性粒细胞趋化因子(ECF-L)同源,但在嗜酸性粒细胞中仅引起弱趋化反应。Ym1与肝素、GlcN低聚物和硫酸乙酰肝素结合。Ym1属于几丁质酶相关的蛋白质家族,但不具有几丁质酶活性。
亚型
一抗
靶抗原
Ym1
别名
β-N-乙酰己糖胺酶Ym1,Chi3l3,几丁质酶样蛋白3,ECF-L,嗜酸性粒细胞趋化因子,嗜酸性粒细胞趋化因子-L,分泌蛋白Ym1
活性物种
小鼠
偶联
未偶联的
宿主物种
兔
细胞类型
其它细胞系
种属
小鼠,其它细胞系
应用
流式细胞术,免疫印迹,免疫荧光,免疫组化
研究领域
免疫
克隆
Not applicable
基因编号
12655
同种型
IgG
(A) Flow cyometry analysis of peritoneal macrophages from normal (+/+) and SHIP knockout (-/-) mice. Cells were fixed, permeabilized, and labeled with an isotype control antibody (red) or Anti-Ym1 Antibody, Polyclonal, followed by an Alexa Fluor® 488-conjugated anti-rabbit antibody (blue).
(B) Monocytes isolated from bone marrow of normal and SHIP knockout mice were labeled with Anti-Ym1 Antibody, Polyclonal, followed by an Alexa Fluor® 488-conjugated anti-rabbit antibody.
(C) Western blot analysis of cell lysates from mouse peritoneal macrophages isolated from normal and SHIP knockout mice and probed with Anti-Ym1 Antibody, Polyclonal. Ym1 has a predicted molecular mass of ~45 kDa
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Species | Mouse, Other |
---|---|
Clone | Not applicable |
Gene Id | 12655 |
Alternative Names | beta-N-acetylhexosaminidase Ym1, Chi3l3, chitinase-like protein 3, ECF-L, eosinophil chemotactic cytokine, eosinophil chemotactic factor-L, secreted protein Ym1 |
Isotype | IgG |
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