产品号 #100-1065_C
Mouse monoclonal antibody against human, mouse, rat vimentin
概述
Clone1A7 is a mouse monoclonal antibody that reacts with human vimentin, a class III intermediate filament that is composed of α-helical regions linked into a coiled-coil protein and confers mechanical resistance in cells. Use the 1A7 clone in applications requiring detection of vimentin. It is verified for use with immunocytochemistry (ICC).
Vimentin is expressed in mesenchymal cells and mediates several functions, including cell adhesion, signaling, and migration. Vimentin filaments have been reported to interact with signaling proteins, such as Cdc42, Rac1, and the 14-3-3 family of proteins, suggesting a role in modulation and regulation of signal transduction processes. This structural protein is also involved in the LARP-6 dependent binding and stabilization of collagen mRNA during the development of tissue fibrosis. It is regarded as a marker of mesenchymal-derived cells but has been implicated in tumor detection, such as in distinguishing between some sarcomas and carcinomas.
Subtype
Primary Antibodies
Target Antigen
Vimentin
Alternative Names
VIM, CTRCT30, HEL113
Reactive Species
Human
Conjugation
Unconjugated
Host Species
Mouse
Cell Type
Intestinal Cells
Species
Human
Application
Immunocytochemistry, Immunofluorescence
Area of Interest
Organoids
Clone
1A7
Gene ID
7431
Isotype
IgG1, kappa
Data Figures
Figure 1. Anti-Human Vimentin Antibody, Clone 1A7 is Suitable for Staining Human Intestinal Organoids
H9 human intestinal organoids were cultured using STEMdiff™ Intestinal Organoid Kit (Catalog #05140), then fixed and labeled with Anti-Human Vimentin Antibody, Clone 1A7, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, iFluor™ 568 (Catalog #100-1080). Nuclei were counter-stained with DAPI (blue). Inset shows cells labeled with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21 (Catalog #60070), followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, iFluor™ 568 (with DAPI staining).
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet Product Name
Anti-Human Vimentin Antibody, Clone 1A7 Catalog #
100-1065 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Human Vimentin Antibody, Clone 1A7 Catalog #
100-1065 Lot #
All Language
English Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages
Workflow Stages for Intestinal Epithelial Cell Research
Resources and Publications
Educational Materials (4)
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