抗人Vimentin抗体,克隆Clone 1A7

小鼠单克隆抗体,抗人、小鼠、大鼠Vimentin

产品号 #(选择产品)

产品号 #100-1065_C

小鼠单克隆抗体,抗人、小鼠、大鼠Vimentin

总览

克隆1A7是一种小鼠单克隆抗体,可与人Vimentin反应,Vimentin是一种III类中间丝蛋白,由α-螺旋区域连接形成卷曲螺旋结构,可增强细胞的机械抗性。可在需要检测Vimentin的应用中使用1A7克隆。经验证可用于免疫细胞化学(ICC)。

Vimentin在间充质细胞中表达,并介导多种功能,包括细胞粘附、信号传导和迁移。据报道,Vimentin纤维与Cdc42、Rac1和14-3-3蛋白家族等信号蛋白相互作用,提示其在信号转导过程的调控中发挥作用。这种结构蛋白还参与组织纤维化发展过程中LARP-6依赖性胶原mRNA的结合与稳定。虽然被视为间充质来源细胞的标志物,但该蛋白在肿瘤检测(如某些肉瘤与癌的鉴别诊断)中亦具有重要价值。

亚型
一抗
 
靶抗原
Vimentin
 
别名
VIM,CTRCT30,HEL113
 
活性物种

 
偶联
未偶联的
 
宿主物种
小鼠
 
细胞类型
肠道细胞
 
种属

 
应用
免疫细胞化学,免疫荧光
 
研究领域
类器官
 
克隆
1A7
 
基因编号
7431
 
同种型
IgG1,kappa
 

Data Figures

Figure 1. Anti-Human Vimentin Antibody, Clone 1A7 is Suitable for Staining Human Intestinal Organoids

H9 human intestinal organoids were cultured using STEMdiff™ Intestinal Organoid Kit (Catalog #05140), then fixed and labeled with Anti-Human Vimentin Antibody, Clone 1A7, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, iFluor™ 568 (Catalog #100-1080). Nuclei were counter-stained with DAPI (blue). Inset shows cells labeled with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21 (Catalog #60070), followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, iFluor™ 568 (with DAPI staining).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
100-1065
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
100-1065
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

更多信息

更多信息
Species Human
Clone 1A7
Gene Id 7431
Alternative Names VIM, CTRCT30, HEL113
Isotype IgG1, kappa
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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