产品号 #100-1345_C
兔单克隆抗体,抗人Synaptophysin
兔单克隆抗体(克隆号 249)可识别突触素(synaptophysin),这是一种分子量约为 38 kDa 的完整跨膜蛋白,具有四个跨膜结构域,是突触小泡的主要结构蛋白之一。该重组抗体适用于检测突触素的相关应用,包括免疫组织化学(IHC)、免疫细胞化学(ICC)和免疫荧光(IF)。该抗体克隆已验证可用于标记人多能干细胞(hPSC)来源的前脑型神经元中的突触素阳性位点,这些神经元由 STEMdiff™ 前脑神经元分化试剂盒(产品号 #08600)和 STEMdiff™ 前脑神经元成熟试剂盒(产品号 #08605)生成。
突触素可与多种运动蛋白和突触囊泡蛋白相互作用,包括动力蛋白 I(dynamin I)、肌球蛋白 V、囊泡质子泵 V-ATP 酶以及衔接蛋白 1(AP-1)。它广泛参与突触囊泡的功能调控,如蛋白质分选、囊泡启动、突触形成、胞吐和胞吞等过程。突触素也是评估轴突损伤的重要标志物。研究发现其可与淀粉样前体蛋白(APP)共定位,后者是多发性硬化症患者中轴突损伤的标志物。此外,突触素缺失的基因敲除小鼠表现出空间学习能力下降及新奇物体识别受损的行为表型。
亚型
一抗
靶抗原
SYP
别名
MRX96,MRXSYP,XLID96
活性物种
人
偶联
未偶联的
宿主物种
兔
细胞类型
神经细胞,PSC衍生,神经元
种属
人
应用
免疫细胞化学
研究领域
神经科学,干细胞生物学
克隆
249
基因编号
6855
同种型
IgG
Figure 1. Mature Human Forebrain Neurons Stained with Anti-Human Synaptophysin Antibody, Monoclonal
Forebrain-type neurons were generated from hPSC-derived neural progenitor cells using STEMdiff™ Forebrain Neuron Differentiation Kit for 7 days and subsequently matured for the following 5 weeks using STEMdiff™ Forebrain Neuron Maturation Kit. Mature forebrain neurons were then fixed and labeled with Anti-Human Synaptophysin Antibody, Clone 249, followed by Goat Anti-Rabbit IgG (H+L) Antibody, Polyclonal, iFluor™ 488 (green) (Catalog #100-1082). Nuclei were counterstained with DAPI (blue). Synaptophysin is concentrated as discrete puncta distributed along axonal processes that were labeled with Anti-Beta-Tubulin III Antibody, Clone TUJ1 (red) (Catalog #60052). Inset shows forebrain-type neurons labeled with a rabbit IgG isotype control antibody, followed by Goat Anti-Rabbit IgG (H+L) Antibody, Polyclonal, iFluor™ 488 (with DAPI staining).
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Species | Human |
---|---|
Clone | 249 |
Gene Id | 6855 |
Alternative Names | MRX96, MRXSYP, XLID96 |
Isotype | IgG |
用于将人 ES 和 iPS 细胞衍生的神经前体细胞分化为神经元前体细胞的分化试剂盒
用于从人ES和iPS细胞衍生的神经元前体细胞生成功能性神经元的成熟试剂盒
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