抗人突触蛋白(MRX96)抗体,克隆249

兔单克隆抗体,抗人Synaptophysin

产品号 #(选择产品)

产品号 #100-1345_C

兔单克隆抗体,抗人Synaptophysin

总览

兔单克隆抗体(克隆号 249)可识别突触素(synaptophysin),这是一种分子量约为 38 kDa 的完整跨膜蛋白,具有四个跨膜结构域,是突触小泡的主要结构蛋白之一。该重组抗体适用于检测突触素的相关应用,包括免疫组织化学(IHC)、免疫细胞化学(ICC)和免疫荧光(IF)。该抗体克隆已验证可用于标记人多能干细胞(hPSC)来源的前脑型神经元中的突触素阳性位点,这些神经元由 STEMdiff™ 前脑神经元分化试剂盒(产品号 #08600)和 STEMdiff™ 前脑神经元成熟试剂盒(产品号 #08605)生成。

突触素可与多种运动蛋白和突触囊泡蛋白相互作用,包括动力蛋白 I(dynamin I)、肌球蛋白 V、囊泡质子泵 V-ATP 酶以及衔接蛋白 1(AP-1)。它广泛参与突触囊泡的功能调控,如蛋白质分选、囊泡启动、突触形成、胞吐和胞吞等过程。突触素也是评估轴突损伤的重要标志物。研究发现其可与淀粉样前体蛋白(APP)共定位,后者是多发性硬化症患者中轴突损伤的标志物。此外,突触素缺失的基因敲除小鼠表现出空间学习能力下降及新奇物体识别受损的行为表型。

亚型
一抗
 
靶抗原
SYP
 
别名
MRX96,MRXSYP,XLID96
 
活性物种

 
偶联
未偶联的
 
宿主物种

 
细胞类型
神经细胞,PSC衍生,神经元
 
种属

 
应用
免疫细胞化学
 
研究领域
神经科学,干细胞生物学
 
克隆
249
 
基因编号
6855
 
同种型
IgG
 

Data Figures

Figure 1. Mature Human Forebrain Neurons Stained with Anti-Human Synaptophysin Antibody, Monoclonal

Forebrain-type neurons were generated from hPSC-derived neural progenitor cells using STEMdiff™ Forebrain Neuron Differentiation Kit for 7 days and subsequently matured for the following 5 weeks using STEMdiff™ Forebrain Neuron Maturation Kit. Mature forebrain neurons were then fixed and labeled with Anti-Human Synaptophysin Antibody, Clone 249, followed by Goat Anti-Rabbit IgG (H+L) Antibody, Polyclonal, iFluor™ 488 (green) (Catalog #100-1082). Nuclei were counterstained with DAPI (blue). Synaptophysin is concentrated as discrete puncta distributed along axonal processes that were labeled with Anti-Beta-Tubulin III Antibody, Clone TUJ1 (red) (Catalog #60052). Inset shows forebrain-type neurons labeled with a rabbit IgG isotype control antibody, followed by Goat Anti-Rabbit IgG (H+L) Antibody, Polyclonal, iFluor™ 488 (with DAPI staining).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
100-1345
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
100-1345
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

更多信息

更多信息
Species Human
Clone 249
Gene Id 6855
Alternative Names MRX96, MRXSYP, XLID96
Isotype IgG
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