产品号 #100-1342_C
抗人MAP2的兔多克隆抗体
抗人MAP2的兔多克隆抗体可特异性识别微管相关蛋白 2(MAP2),MAP2是一种参与微管稳定和树突发育分支的蛋白质。该多克隆抗体可用于需要检测MAP2的应用,例如免疫组织化学(IHC)、免疫细胞化学(ICC)和免疫荧光(IF)。该抗体克隆已通过验证,可标记使用STEMdiff™前脑神经元分化试剂盒(产品号 #08600)和STEMdiff™前脑神经元成熟试剂盒(产品号 #08605)生成的MAP2阳性hPSC来源前脑型神经元。MAP2属于神经元中最丰富的微管相关蛋白家族。在啮齿动物中,通过基因可变剪接产生三种亚型:MAP2A、MAP2B和MAP2C。MAP2C可特异性调控树突早期发育;随着时间的推移,它们会被MAP2取代。MAP2的选择性剪接与多种神经退行性疾病(例如亨廷顿氏病)的发展有关。此外,患有传染性海绵状脑病(TSE),例如致死性家族失眠症(FFI)和克雅氏病的患者,其MAP2表达水平几乎检测不到,这表明MAP2的下降可能是TSE发生的标志性特征。
亚型
一抗
靶抗原
MAP2 (Microtubule-Associated Protein-2)
别名
MAP-2,MAP2A,MAP2B,MAP2C
活性物种
人
偶联
未偶联的
宿主物种
兔
细胞类型
神经细胞,PSC衍生,神经元
种属
人
应用
免疫细胞化学
研究领域
神经科学,干细胞生物学
克隆
Poly
基因编号
4133
同种型
Not applicable
Figure 1. Mature Human Forebrain Neurons Stained with Anti-Human MAP2 Antibody, Polyclonal
Forebrain-type neurons were generated from hPSC-derived neural progenitor cells using STEMdiff™ Forebrain Neuron Differentiation Kit for 7 days and subsequently matured for the following 5 weeks using STEMdiff™ Forebrain Neuron Maturation Kit. Mature forebrain neurons were then fixed and labeled with Anti-Human MAP2 Antibody, followed by Goat Anti-Rabbit IgG (H+L) Antibody, Polyclonal, iFluor™ 488 (green) (Catalog #100-1082). Co-staining with Anti-Beta-Tubulin III Antibody, Clone TUJ1 (red) (Catalog #60052) shows the Anti-Human MAP2 Antibody is specific for mature neurons. Nuclei were counterstained with DAPI (blue). Inset shows forebrain-type neurons labeled with a rabbit IgG isotype control antibody, followed by Goat Anti-Rabbit IgG (H+L) Antibody, Polyclonal, iFluor™ 488 (with DAPI staining).
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Species | Human |
---|---|
Clone | Poly |
Gene Id | 4133 |
Alternative Names | MAP-2, MAP2A, MAP2B, MAP2C |
用于将人 ES 和 iPS 细胞衍生的神经前体细胞分化为神经元前体细胞的分化试剂盒
用于从人ES和iPS细胞衍生的神经元前体细胞生成功能性神经元的成熟试剂盒
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