抗人MAP2(MAP-2)抗体,多克隆

抗人MAP2的兔多克隆抗体

产品号 #(选择产品)

产品号 #100-1342_C

抗人MAP2的兔多克隆抗体

总览

抗人MAP2的兔多克隆抗体可特异性识别微管相关蛋白 2(MAP2),MAP2是一种参与微管稳定和树突发育分支的蛋白质。该多克隆抗体可用于需要检测MAP2的应用,例如免疫组织化学(IHC)、免疫细胞化学(ICC)和免疫荧光(IF)。该抗体克隆已通过验证,可标记使用STEMdiff™前脑神经元分化试剂盒(产品号 #08600)和STEMdiff™前脑神经元成熟试剂盒(产品号 #08605)生成的MAP2阳性hPSC来源前脑型神经元。MAP2属于神经元中最丰富的微管相关蛋白家族。在啮齿动物中,通过基因可变剪接产生三种亚型:MAP2A、MAP2B和MAP2C。MAP2C可特异性调控树突早期发育;随着时间的推移,它们会被MAP2取代。MAP2的选择性剪接与多种神经退行性疾病(例如亨廷顿氏病)的发展有关。此外,患有传染性海绵状脑病(TSE),例如致死性家族失眠症(FFI)和克雅氏病的患者,其MAP2表达水平几乎检测不到,这表明MAP2的下降可能是TSE发生的标志性特征。

亚型
一抗
 
靶抗原
MAP2 (Microtubule-Associated Protein-2)
 
别名
MAP-2,MAP2A,MAP2B,MAP2C
 
活性物种

 
偶联
未偶联的
 
宿主物种

 
细胞类型
神经细胞,PSC衍生,神经元
 
种属

 
应用
免疫细胞化学
 
研究领域
神经科学,干细胞生物学
 
克隆
Poly
 
基因编号
4133
 
同种型
Not applicable
 

Data Figures

Figure 1. Mature Human Forebrain Neurons Stained with Anti-Human MAP2 Antibody, Polyclonal

Forebrain-type neurons were generated from hPSC-derived neural progenitor cells using STEMdiff™ Forebrain Neuron Differentiation Kit for 7 days and subsequently matured for the following 5 weeks using STEMdiff™ Forebrain Neuron Maturation Kit. Mature forebrain neurons were then fixed and labeled with Anti-Human MAP2 Antibody, followed by Goat Anti-Rabbit IgG (H+L) Antibody, Polyclonal, iFluor™ 488 (green) (Catalog #100-1082). Co-staining with Anti-Beta-Tubulin III Antibody, Clone TUJ1 (red) (Catalog #60052) shows the Anti-Human MAP2 Antibody is specific for mature neurons. Nuclei were counterstained with DAPI (blue). Inset shows forebrain-type neurons labeled with a rabbit IgG isotype control antibody, followed by Goat Anti-Rabbit IgG (H+L) Antibody, Polyclonal, iFluor™ 488 (with DAPI staining).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
100-1342
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
100-1342
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

更多信息

更多信息
Species Human
Clone Poly
Gene Id 4133
Alternative Names MAP-2, MAP2A, MAP2B, MAP2C
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