产品号 #100-1064_C
兔多克隆抗体,抗人、小鼠、大鼠细胞角蛋白20
该兔多克隆抗体与人、小鼠和大鼠细胞角蛋白20反应,这是一种低分子量的I型细胞角蛋白,是成熟肠细胞和杯状细胞的主要成分。细胞角蛋白20主要在胃肠道(GI)上皮、尿路上皮和Merkel细胞中表达,并作为胃肠道分化的标志。该多克隆抗体适用于需要检测细胞角蛋白20的实验应用,如蛋白印迹(WB)、免疫组化(IHC-P)或免疫荧光(IF),并已验证可用于免疫细胞化学(ICC)实验。细胞角蛋白20负责维持上皮细胞的结构完整性,并在肠道上皮中维持中间丝的有序排列。细胞角蛋白20也是胰腺癌和结直肠癌的重要标志物,在结肠、胰腺、胃和胆道腺癌中均有检测到。细胞角蛋白20的表达也与乳腺癌的进展和转移有关,表明其可能作为预测乳腺癌预后的生物标志物。
亚型
一抗
靶抗原
Cytokeratin 20
别名
CK20,K20,KRT20,角蛋白20
活性物种
人
偶联
未偶联的
宿主物种
兔
细胞类型
肠道细胞
种属
人,小鼠,大鼠
应用
免疫细胞化学,免疫荧光,免疫组化,Western印迹
研究领域
类器官
克隆
Not applicable
基因编号
54474
同种型
Not applicable
Figure 1. Anti-Human Cytokeratin 20 Antibody, Polyclonal is Suitable for Staining Human Intestinal Organoids
H9 human intestinal organoids were cultured using STEMdiff™ Intestinal Organoid Kit (Catalog #05140), then fixed and labeled with Anti-Human Cytokeratin 20 Antibody, Polyclonal, followed by Goat Anti-Rabbit IgG (H+L) Antibody, Polyclonal, iFluor™ 488 (Catalog #100-1082). Nuclei were counter-stained with DAPI (blue). Inset shows cells labeled with a rabbit IgG isotype control antibody, followed by Goat Anti-Rabbit IgG (H+L) Antibody, Polyclonal, iFluor™ 488 (with DAPI staining).
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
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Species | Human, Mouse, Rat |
---|---|
Clone | Not applicable |
Gene Id | 54474 |
Alternative Names | CK20, K20, KRT20, keratin 20 |
Isotype | Not applicable |
DNA标记染料
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