产品号 #60158_C
Mouse monoclonal IgG1 antibody against human, rhesus, cynomolgus CD25
概述
The BC96 antibody reacts with human CD25, a 55 kDa type I integral transmembrane glycoprotein. CD25 is involved in the activation and proliferation of lymphocytes and can be used in the identification of T cell subsets in viral infections like HIV and HTLV. CD25 is present on activated T and B cells, monocytes, macrophages, and regulatory T cells. Expression of CD25, together with CD4 and FOXP3, is considered a phenotypic signature for regulatory T cells. CD25 has a low affinity for its IL-2 ligand but associates with IL-2 receptor β (CD122) and common γ (CD132) chains to form a high affinity IL-2 receptor.
Subtype
Primary Antibodies
Target Antigen
CD25
Alternative Names
IL-2 Receptor alpha chain, IL-2Ra, IL2Ra, Tac, p55
Reactive Species
Baboon, Chimpanzee, Cynomolgus, Human, Macaque, Rhesus
Conjugation
Alexa Fluor 488, APC, Biotin, FITC, PE, Unconjugated
Host Species
Mouse
Cell Type
B Cells, T Cells, T Cells, Regulatory
Application
Flow Cytometry
Area of Interest
Cancer, Immunology, Transplantation Research, Cell Therapy Development
Clone
BC96
Gene ID
3559
Isotype
IgG1, kappa
Data Figures
Flow cytometry analysis of PHA-L-activated human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD25 Antibody, Clone BC96, followed by a rat anti-mouse IgG1 antibody, FITC (filled histogram), or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21 (Catalog #60070), followed by a rat anti-mouse IgG1 antibody, FITC (solid line histogram).
Flow cytometry analysis of PHA-L-activated human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD25 Antibody, Clone BC96, FITC (filled histogram), or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, FITC (Catalog #60070FI; solid line histogram).
Flow cytometry analysis of PHA-L-activated human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD25 Antibody, Clone BC96, PE (filled histogram), or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, PE (Catalog #60070PE; solid line histogram).
Flow cytometry analysis of PHA-L-activated human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD25 Antibody, Clone BC96, APC (filled histogram), or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, APC (Catalog #60070AZ; solid line histogram).
Flow cytometry analysis of PHA-L-activated human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD25 Antibody, Clone BC96, Alexa Fluor® 488 (filled histogram), or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, Alexa Fluor® 488 (Catalog #60070AD; solid line histogram).
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet Product Name
Anti-Human CD25 Antibody, Clone BC96, APC Catalog #
60158AZ.1, 60158AZ Lot #
All Language
English Document Type
Product Information Sheet Product Name
Anti-Human CD25 Antibody, Clone BC96, Biotin Catalog #
60158BT Lot #
All Language
English Document Type
Product Information Sheet Product Name
Anti-Human CD25 Antibody, Clone BC96, PE Catalog #
60158PE, 60158PE.1 Lot #
All Language
English Document Type
Product Information Sheet Product Name
Anti-Human CD25 Antibody, Clone BC96, FITC Catalog #
60158FI.1, 60158FI Lot #
All Language
English Document Type
Product Information Sheet Product Name
Anti-Human CD25 Antibody, Clone BC96 Catalog #
60158 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Human CD25 Antibody, Clone BC96, APC Catalog #
60158AZ.1, 60158AZ Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Human CD25 Antibody, Clone BC96, Biotin Catalog #
60158BT Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Human CD25 Antibody, Clone BC96, PE Catalog #
60158PE, 60158PE.1 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Human CD25 Antibody, Clone BC96, FITC Catalog #
60158FI.1, 60158FI Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Human CD25 Antibody, Clone BC96 Catalog #
60158 Lot #
All Language
English Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages
Workflow Stages for B Cell Research
Resources and Publications
Educational Materials (2)
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Legal Statement: Alexa Fluor is a registered trademark of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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