Agar Leukocyte Conditioned Medium

Source of colony-stimulating factors for assays of human hematopoietic progenitor cells

产品号 #02300_C

Source of colony-stimulating factors for assays of human hematopoietic progenitor cells

概述

Agar Leukocyte Conditioned Medium (Agar-LCM) is a source of colony-stimulating factors for assays of hematopoietic progenitor cells from light-density fractions of human bone marrow and peripheral blood. It is suitable for the growth of colony-forming unit erythroid (CFU­E), burst-forming unit-erythroid (BFU-E), colony-forming unit-macrophage (CFU-M), colony-forming unit-granulocyte (CFU-G), colony-forming unit-granulocyte, macrophage (CFU-GM) and colony-forming unit-granulocyte, erythroid, macrophage, megakaryocyte (CFU-GEMM) when added to MethoCult™ medium at a final concentration of 10% with the addition of erythropoietin (EPO; Catalog #02625).

Agar-LCM is prepared using normal human peripheral blood leukocytes in an agar-containing medium.

Contains
• Agar Leukocyte Conditioned Medium <br> • Fetal bovine serum (10%) <br> • Iscove's MDM <br> • 1 x 10^4 2-Mercaptoethanol (ME)
 
Subtype
Basal Media
 
Cell Type
Hematopoietic Stem and Progenitor Cells
 
Species
Human
 
Application
Cell Culture
 
Area of Interest
Stem Cell Biology
 

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
02300
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
02300
Lot #
All
Language
English

Applications

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Resources and Publications

Educational Materials (1)

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