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EasySep™ Rat B Cell Isolation Kit

Immunomagnetic negative selection kit
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产品号 #19644_C

Immunomagnetic negative selection kit

产品优势


  • Fast, easy-to-use and column-free

  • Up to 95% purity

  • Isolated cells are untouched

产品组分包括

    • EasySep™ Rat B Cell Isolation Cocktail, 1 mL
    • EasySep™ Dextran RapidSpheres™ 50102, 1 mL
    • EasySep™ Rat B Cell Isolation Cocktail, 1 mL
    • EasySep™ Dextran RapidSpheres™ 50102, 1 mL
    • RoboSep™ Buffer (Catalog #20104)
    • RoboSep™ Filter Tips (Catalog #20125)
main product photo
EasySep™ Rat B Cell Isolation Kit
Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.
  1. EasySep™ Magnet
    EasySep™ Magnet

    Magnet for column-free immunomagnetic separation

  2. EasySep™ Buffer
    EasySep™ Buffer

    Cell separation buffer

概述
实验数据
产品说明书及文档
相关材料与文献

概述

The EasySep™ Rat B Cell Isolation Kit is designed to isolate B cells from single-cell suspensions of splenocytes or lymph nodes or from whole blood by negative selection. The EasySep™ procedure involves labeling unwanted cells with antibody complexes and magnetic particles. The magnetically labeled cells are separated from the untouched desired cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube.

Magnet Compatibility
<div> • EasySep™ Magnet (Catalog #18000) </div> <div> • ”The BigEasy” EasySep™ Magnet (Catalog #18001) </div> <div> • Easy50 EasySep™ Magnet (Catalog #18002) </div> <div> • EasyEights™ EasySep™ Magnet (Catalog #18103) </div> <div> • RoboSep™-S (Catalog #21000)
 
Subtype
Cell Isolation Kits
 
Cell Type
B Cells
 
Species
Rat
 
Sample Source
Other, Spleen, Whole Blood
 
Selection Method
Negative
 
Application
Cell Isolation
 
Brand
EasySep, RoboSep
 
Area of Interest
Immunology
 

Data Figures

19644 FACS

Starting with a single-cell suspension of rat splenocytes, the B cell content (CD45RA+CD3-) of the isolated fraction is typically 94.1 ± 3.8% (mean ± SD using the purple EasySep™ Magnet). In the example above, the purities of the start and final isolated fractions are 44.5% and 95.6%, respectively.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
EasySep™ Rat B Cell Isolation Kit
Catalog #
19644
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
RoboSep™ Rat B Cell Isolation Kit
Catalog #
19644RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Rat B Cell Isolation Kit
Catalog #
19644
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Rat B Cell Isolation Kit
Catalog #
19644
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
RoboSep™ Rat B Cell Isolation Kit
Catalog #
19644RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
RoboSep™ Rat B Cell Isolation Kit
Catalog #
19644RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
RoboSep™ Rat B Cell Isolation Kit
Catalog #
19644RF
Lot #
All
Language
English
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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