The EasySep™ Human CD33 Positive Selection Kit II is designed to isolate CD33+ cells from ammonium chloride lysed blood or bone marrow, or from nucleated cells prepared by density separation with RosetteSep™ DM-M density medium (Catalog #15725) by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD33 and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD33 antigen is expressed on cells of the myeloid lineage.
This product replaces the EasySep™ Human CD33 Positive Selection Kit (Catalog #18257) for even faster cell isolations.
Cell Type Granulocytes and Subsets, Monocytes, Myeloid Cells
Species Human
Sample Source PMNC
Selection Method Positive
Brand EasySep
Area of Interest Chimerism, HLA, Immunology
Data Figures
Starting with ammonium chloride-lysed peripheral blood, the CD33+ cell content of the isolated fraction is typically 95.6 ± 1.6%, as assessed by labeling with CD14 and CD66b (mean + SD using “The Big Easy” EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 44.7% and 96.3%, respectively.
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
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