产品号 #18002_C
Magnet for column-free immunomagnetic separation
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概述
Perform magnetic cell separation procedures easily and efficiently using the Easy 50 EasySep™ Magnet with EasySep™ cell isolation reagents. The Easy 50 EasySep™ Magnet generates a high-gradient magnetic field in the interior cavity that is strong enough to separate cells labeled with magnetic EasySep™ particles without the use of columns. The Easy 50 EasySep™ Magnet is designed to hold a standard 50 mL conical tube, enabling you to process up to 4 x 109 cells in one isolation procedure.
Not sure which magnet to use? Visit our EasySep™ Cell Separation Magnets page to compare the different options and select the appropriate magnet for your research.
Learn more about how immunomagnetic EasySep™ technology works.
Species
Human, Mouse, Non-Human Primate, Other
Application
Cell Isolation
Brand
EasySep
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet Product Name
Easy 50 EasySep™ Magnet Catalog #
18002 Lot #
All Language
English Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages
Workflow Stages for B Cell Research
Workflow Stages for Dendritic Cell Research
Workflow Stages for Granulocyte Research
Workflow Stages for Total Lymphocyte Research
Workflow Stages for Monocyte Research
Workflow Stages for Myeloid Cell Research
Workflow Stages for Natural Killer Cell Research
Workflow Stages for T Cell Research
Workflow Stages for HLA Analysis
Workflow Stages for T Cell Engineering
Workflow Stages for Chimerism Analysis
Resources and Publications
Educational Materials (5)
Frequently Asked Questions
Can EasySep™ be used for either positive or negative selection?
Yes. The EasySep™ kits use either a negative selection approach by targeting and removing unwanted cells or a positive selection approach targeting desired cells. Depletion kits are also available for the removal of cells with a specific undesired marker (e.g. GlyA).
How does the separation work?
Magnetic particles are crosslinked to cells using Tetrameric Antibody Complexes (TAC). When placed in the EasySep™ Magnet, labeled cells migrate to the wall of the tube. The unlabeled cells are then poured off into a separate fraction.
Which columns do I use?
The EasySep™ procedure is column-free. That's right - no columns!
How can I analyze the purity of my enriched sample?
The Product Information Sheet provided with each EasySep™ kit contains detailed staining information.
Can EasySep™ separations be automated?
Yes. RoboSep™, the fully automated cell separator, automates all EasySep™ labeling and cell separation steps.
Can EasySep™ be used to isolate rare cells?
Yes. We recommend a cell concentration of 2x108 cells/mL and a minimum working volume of 100 µL. Samples containing 2x107 cells or fewer should be suspended in 100 µL of buffer.
Are the EasySep™ magnetic particles FACS-compatible?
Yes, the EasySep™ particles are flow cytometry-compatible, as they are very uniform in size and about 5000X smaller than other commercially available magnetic beads used with column-free systems.
Can the EasySep™ magnetic particles be removed after enrichment?
No, but due to the small size of these particles, they will not interfere with downstream applications.
How does the binding of the EasySep™ magnetic particle affect the cells? is the function of positively selected cells altered by the bound particles?
Hundreds of publications have used cells selected with EasySep™ positive selection kits for functional studies. Our in-house experiments also confirm that selected cells are not functionally altered by the EasySep™ magnetic particles.
If particle binding is a key concern, we offer two options for negative selection. The EasySep™ negative selection kits can isolate untouched cells with comparable purities, while RosetteSep™ can isolate untouched cells directly from whole blood without using particles or magnets.
If particle binding is a key concern, we offer two options for negative selection. The EasySep™ negative selection kits can isolate untouched cells with comparable purities, while RosetteSep™ can isolate untouched cells directly from whole blood without using particles or magnets.
Can I alter the separation time in the magnet?
Yes; however, this may impact the kit's performance. The provided EasySep™ protocols have already been optimized to balance purity, recovery and time spent on the isolation.
For positive selection, can I perform more than 3 separations to increase purity?
Yes, the purity of targeted cells will increase with additional rounds of separations; however, cell recovery will decrease.
Which cell separation kits are compatible with the "Easy 50" EASYSEP™ magnet?
At present, the "Easy 50" EasySep™ magnet is only compatible with EasySep™ kits for human cell separation.
T Cells: 19051 (T Cells), 19052 (CD4 T cells), 19157 (Memory CD4 T Cells), 19053 (CD8 T Cells), 19159 (Memory CD8 T Cells - please contact Tech Support)
B Cells: 19054 (B Cells), 19254 (Naïve B cells)
Other Cell Types: 19055 (NK Cells), 19058 (Monocytes without CD16 depletion), 19059 (Monocytes), 19062 (Plasmacytoid DCs), 19251 (pan-DCs)
For HLA Analysis: 19951HLA (T Cells from whole blood), 19954HLA (B Cells from whole blood), 19961HLA (Total lymphocytes from whole blood)
T Cells: 19051 (T Cells), 19052 (CD4 T cells), 19157 (Memory CD4 T Cells), 19053 (CD8 T Cells), 19159 (Memory CD8 T Cells - please contact Tech Support)
B Cells: 19054 (B Cells), 19254 (Naïve B cells)
Other Cell Types: 19055 (NK Cells), 19058 (Monocytes without CD16 depletion), 19059 (Monocytes), 19062 (Plasmacytoid DCs), 19251 (pan-DCs)
For HLA Analysis: 19951HLA (T Cells from whole blood), 19954HLA (B Cells from whole blood), 19961HLA (Total lymphocytes from whole blood)
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