Easily and efficiently scale up your manual cell isolations from large-volume samples, such as leukopaks and whole blood. Rather than splitting your cell suspension and performing multiple rounds of isolations, with the Easy 250 EasySep™ Magnet, you can process up to 225 mL and 1.25 x 1010cells in a single isolation.
Use the Easy 250 EasySep™ Magnet with a standard T-75 cm2 cell culture flask and EasySep™ reagents to separate cells from large-volume samples without the use of columns. With its strong, high-gradient internal magnetic field, the magnet isolates cells labeled with magnetic EasySep™ particles, allowing you to simply pipette out the unlabeled cell fraction and obtain cells that are immediately ready for downstream applications. Save time and obtain highly purified cells with EasySep™ column-free technology.
Not sure which magnet to use? Visit our EasySep™ Cell Separation Magnets page to compare the different options and select the appropriate magnet for your research.
Figure 1. Using the Easy 250 EasySep™ Magnet Results in the Isolation of Highly Purified Cells of Interest
Human immune cells were isolated from processed leukopaks using the corresponding (A) EasySep™ negative selection kits or (B) EasySep™ positive selection kits (see Table 1). Cell purity was measured before isolation (Start) and after isolation with the Easy 250 EasySep™ Magnet (Easy 250), EasySep™ Magnet (Purple) or “The Big Easy” EasySep™ Magnet (Silver). Purity was assessed by staining with cell surface markers for pan-T cells (CD3+), CD4+ T cells (CD3+CD4+), CD8+ T cells (CD3+CD8+), or monocytes (CD14+CD45+), and analyzed by flow cytometry. Data shown as mean ± SD; n = 4 - 12.
Figure 2. Easy 250 EasySep™ Magnet Can Be Used to Isolate Mononuclear Cells from Whole Blood and Leukopak Samples of Up to 125 mL
Mononuclear cells (MNCs) were isolated using the EasySep™ Direct Human PBMC Isolation Kit (Catalog #19654) from large whole blood samples (25 - 125 mL) or unprocessed leukopaks (25 - 125 mL) with the Easy 250 EasySep™ Magnet (Easy 250) and from small samples (1 mL whole blood) with the EasySep™ Magnet (Purple). (A) Representative flow cytometry plots show the MNC fraction (Glycophorin A- CD42b-). In the above example, the mononuclear cell content of the whole blood start sample (lysed by ammonium chloride) and non-lysed final isolated fraction is 28.9% and 97.1% (not gated on CD45), respectively. (B) Cell purity was measured before (Start) and after isolation (Easy 250, Purple) based on viable cells. Cells were counted and analyzed by flow cytometry. Data shown as mean ± SD; n = 7 - 9.
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
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