产品号 #60026_C
Mouse monoclonal IgG1 antibody against dextran
概述
The DX1 antibody reacts with dextran, a natural polysaccharide consisting of linear chains of alpha-1,6-linked D-glucopyranose residues with short side chains (mostly 1 - 2 glucose units) 1,3- or 1,4-linked to the backbone of the biopolymer. Dextran has several uses as an additive in food, in lacquers, as a plasma volume expander, and as a coating for particles used in bioimaging or cell separation applications. The DX1 antibody was raised against isomaltotetraose (four sugar units in length) but binds better to longer polymer chains. It is also known to bind to amino-dextran, though with lower affinity. This antibody can be used to detect cells labelled with dextran-coated nanoparticles during StemSep™ and EasySep™ cell separation procedures or for other applications that require the detection of dextran.
This antibody clone has been verified for purity assessments of cells isolated with EasySep™ kits, including EasySep™ Human T Cell Enrichment Kit (Catalog #19051) and EasySep™ Human CD4+ T Cell Enrichment Kit (Catalog #19052).
Subtype
Primary Antibodies
Target Antigen
Dextran
Alternative Names
Not applicable
Conjugation
Unconjugated
Host Species
Mouse
Cell Type
Other
Species
Human, Mouse, Non-Human Primate, Other, Rat
Application
Cell Isolation, ELISA, Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence
Area of Interest
Immunology
Clone
DX1
Gene ID
Not applicable
Isotype
IgG1, kappa
Data Figures
Figure 1. Data for Unconjugated
(A) Competitive ELISA showing effect of free dextran (filled circles) or sucrose (empty circles) on the binding of Anti-Dextran Antibody, Clone DX1 to immobilized dextran. (B) Western blot analysis of a dextran-conjugated carrier protein with Anti-Dextran Antibody, Clone DX1. Lane 1, unconjugated carrier protein (negative control); lane 2, carrier protein after conjugation to dextran.
Figure 2. Data for FITC-Conjugated
Flow cytometry analysis of C57BL/6 mouse bone marrow cells processed with the EasySep™ Mouse CD11b Positive Selection Kit (Catalog #18770) and labeled with Anti-Dextran, Clone DX1, FITC (filled histogram) or a mouse IgG1, kappa FITC isotype control antibody (open histogram).
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet Product Name
Anti-Dextran Antibody, Clone DX1 Catalog #
60026 Lot #
All Language
English Document Type
Product Information Sheet Product Name
Anti-Dextran Antibody, Clone DX1, FITC Catalog #
60026FI.1 Lot #
All Language
English Document Type
Product Information Sheet Product Name
Anti-Dextran Antibody, Clone DX1, FITC Catalog #
100-1575 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Dextran Antibody, Clone DX1 Catalog #
60026 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Dextran Antibody, Clone DX1, FITC Catalog #
60026FI.1, 100-1575 Lot #
All Language
English Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages
Workflow Stages for Chimerism Analysis
Resources and Publications
Educational Materials (3)
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