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ACCUTASE™

Cell detachment solution
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产品号 #07920_C

Cell detachment solution

产品优势


  • Obtain cleaner cultures with a ready-to-use solution, free from mammalian and bacterial-derived products

  • Achieve efficient cell detachment with this gentle trypsin alternative

  • Ensure high cell viability, without the need for a neutralizing solution

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ACCUTASE™
概述
产品说明书及文档
应用领域
相关材料与文献

概述

Use ACCUTASE™ for routine cell detachment from plasticware. This solution of proteolytic and collagenolytic enzymes comes ready-to-use and has been shown to work effectively across a wide variety of cell types. Formulated at a lower concentration than conventional trypsin, this gentle alternative preserves cell surface epitopes for downstream flow cytometry analyses and ensures high cell viability, without the need for a neutralizing solution. ACCUTASE™ does not contain mammalian or bacterial-derived products. Each lot of ACCUTASE™ is tested for sterility (by USP membrane filtration method), enzymatic activity (tested with synthetic chromagenic tetrapeptides), and cell detachment from tissue culture plastic.

Contains
• 1X ACCUTASE™ enzymes in Dulbecco’s phosphate-buffered saline (PBS) <br> • 0.5 mM EDTA•4Na <br> • 3 mg/L Phenol red
 
Subtype
Enzymatic
 
Cell Type
Neural Cells, PSC-Derived, Pluripotent Stem Cells
 
Species
Human, Mouse, Non-Human Primate, Other, Rat
 
Application
Cell Culture
 
Area of Interest
Neuroscience, Stem Cell Biology
 

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
ACCUTASE™
Catalog #
07922, 07920
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
ACCUTASE™
Catalog #
07922, 07920
Lot #
All
Language
English

Resources and Publications

Educational Materials (3)

Maximize Your Pluripotential with the TeSR™ Family of hPSC Culture Media
Brochure
Maximize Your Pluripotential with the TeSR™ Family of hPSC Culture Media
NeuroCult™: Reagents for Brain Tumor Stem Cell Research
Brochure
NeuroCult™: Reagents for Brain Tumor Stem Cell Research
Single-Cell RNA Sequencing Analysis of Regionally Patterned Human Pluripotent Stem Cell-Derived Neural Organoids
Scientific Poster
Single-Cell RNA Sequencing Analysis of Regionally Patterned Human Pluripotent Stem Cell-Derived Neur...

Publications (53)

Transcription Factor-Mediated Differentiation of Human iPSCs into Neurons. M. S. Fernandopulle et al. Current protocols in cell biology 2018 JUN

Abstract

Accurate modeling of human neuronal cell biology has been a long-standing challenge. However, methods to differentiate human induced pluripotent stem cells (iPSCs) to neurons have recently provided experimentally tractable cell models. Numerous methods that use small molecules to direct iPSCs into neuronal lineages have arisen in recent years. Unfortunately, these methods entail numerous challenges, including poor efficiency, variable cell type heterogeneity, and lengthy, expensive differentiation procedures. We recently developed a new method to generate stable transgenic lines of human iPSCs with doxycycline-inducible transcription factors at safe-harbor loci. Using a simple two-step protocol, these lines can be inducibly differentiated into either cortical (i3 Neurons) or lower motor neurons (i3 LMN) in a rapid, efficient, and scalable manner (Wang et al., 2017). In this manuscript, we describe a set of protocols to assist investigators in the culture and genetic engineering of iPSC lines to enable transcription factor-mediated differentiation of iPSCs into i3 Neurons or i3 LMNs, and we present neuronal culture conditions for various experimental applications. {\textcopyright} 2018 by John Wiley & Sons, Inc.
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Substrate stiffness modulates the multipotency of human neural crest derived ectomesenchymal stem cells via CD44 mediated PDGFR signaling. A. Srinivasan et al. Biomaterials 2018 JUN

Abstract

Mesenchymal stem cells (MSCs) have been isolated from various mesodermal and ectodermal tissues. While the phenotypic and functional heterogeneity of MSCs stemming from their developmental origins has been acknowledged, the genetic and environmental factors underpinning these differences are not well-understood. Here, we investigated whether substrate stiffness mediated mechanical cues can directly modulate the development of ectodermal MSCs (eMSCs) from a precursor human neural crest stem cell (NCSC) population. We showed that NCSC-derived eMSCs were transcriptionally and functionally distinct from mesodermal bone marrow MSCs. eMSCs derived on lower substrate stiffness specifically increased their expression of the MSC marker, CD44 in a Rho-ROCK signaling dependent manner, which resulted in a concomitant increase in the eMSCs' adipogenic and chondrogenic differentiation potential. This mechanically-induced effect can only be maintained for short-term upon switching back to a stiff substrate but can be sustained for longer-term when the eMSCs were exclusively maintained on soft substrates. We also discovered that CD44 expression modulated eMSC self-renewal and multipotency via the downregulation of downstream platelet-derived growth factor receptor beta (PDGFRbeta$) signaling. This is the first instance demonstrating that substrate stiffness not only influences the differentiation trajectories of MSCs but also their derivation from upstream progenitors, such as NCSCs.
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Patch-Seq Protocol to Analyze the Electrophysiology, Morphology and Transcriptome of Whole Single Neurons Derived From Human Pluripotent Stem Cells M. van den Hurk et al. Frontiers in Molecular Neuroscience 2018

Abstract

The human brain is composed of a complex assembly of about 171 billion heterogeneous cellular units (86 billion neurons and 85 billion non-neuronal glia cells). A comprehensive description of brain cells is necessary to understand the nervous system in health and disease. Recently, advances in genomics have permitted the accurate analysis of the full transcriptome of single cells (scRNA-seq). We have built upon such technical progress to combine scRNA-seq with patch-clamping electrophysiological recording and morphological analysis of single human neurons in vitro. This new powerful method, referred to as Patch-seq, enables a thorough, multimodal profiling of neurons and permits us to expose the links between functional properties, morphology, and gene expression. Here, we present a detailed Patch-seq protocol for isolating single neurons from in vitro neuronal cultures. We have validated the Patch-seq whole-transcriptome profiling method with human neurons generated from embryonic and induced pluripotent stem cells (ESCs/iPSCs) derived from healthy subjects, but the procedure may be applied to any kind of cell type in vitro. Patch-seq may be used on neurons in vitro to profile cell types and states in depth to unravel the human molecular basis of neuronal diversity and investigate the cellular mechanisms underlying brain disorders.
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Legal Statement: ACCUTASE is a trademark of Innovative Cell Technologies, Inc. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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