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StemSpan™髓系扩增补品（100X）

人粒细胞扩增和分化的无血清培养补充物
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产品号 #02693_C

人粒细胞扩增和分化的无血清培养补充物

产品优势

  • 在14天的液体培养中，促进每个输入CD34+人CB细胞产生数千个CD15+粒细胞。
  • 作为100X浓缩物供应。在解冻和混合后，试管内容物可以直接添加到任何选择的造血细胞扩增培养基中。
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概述

StemSpan™髓系扩增补充（100X）含有重组人细胞因子的组合，用于选择性地促进人髓系祖细胞的扩增和分化，从人脐带血（CB）或骨髓（BM）样本中分离的CD34+细胞产生大量CD15+粒细胞。

StemSpan™髓系扩增补剂可与以下任何StemSpan™培养基结合使用：
•StemSpan™SFEM（目录#09600）
StemSpan™SFEM II（目录#09605）
StemSpan™-XF（目录#100-0073）
StemSpan™-AOF（目录#100-0130）

当添加到无血清培养基中时，StemSpan™髓系扩增补充剂通常促进每个输入CD34+细胞在CD34+人CB细胞启动的14天液体培养中产生数千个CD15+粒细胞。请参阅data选项卡了解更多详细信息。

Contains
• Recombinant human stem cell factor (SCF)
• Recombinant human thrombopoietin (TPO)
• Recombinant human granulocyte colony-stimulating factor (G-CSF)
• Recombinant human granulocyte/macrophage colony-stimulating factor (GM-CSF)
 
Subtype
Supplements
 
Cell Type
Hematopoietic Stem and Progenitor Cells, Myeloid Cells
 
Species
Human
 
Application
Cell Culture, Differentiation, Expansion
 
Brand
StemSpan
 
Area of Interest
Stem Cell Biology, Transplantation Research
 
Formulation Category
Serum-Free
 

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Data Figures

Production of CD13+ and CD15+ Myeloid Cells by the Expansion and Lineage-Specific Differentiation of Human BM-Derived Cells Cultured in StemSpan™ SFEM II Containing Myeloid CD34+ Expansion Supplement

Figure 1. Production of CD13+ and CD15+ Myeloid Cells by the Expansion and Lineage-Specific Differentiation of Human BM-Derived Cells Cultured in StemSpan™ SFEM II Containing Myeloid Expansion Supplement

Flow cytometry dot plots for 14-day cultures of human BM-derived CD34+ cells in StemSpan™ SFEM II containing Myeloid Expansion Supplement showing expression of the HSPC marker CD34, and pan-hematopoietic marker CD45 (A) before and (B) after 14 days of culture, and expression of myeloid markers CD13 and CD15 (C) after 14 days of culture. The frequency of CD34+ cells declined from 83% before culture to ~1% after 14 days. In parallel, myeloid CD13+CD15+ cells gradually accumulated from <10% on day 0 to 46% by day 14. The frequency of CD14+ cells after culture was low (typically <10%, see Table 1).

Table 1. Production of Myeloid Cells from Human CB-Derived CD34+ Cells Cultured in StemSpan™ SFEM II Containing Myeloid Expansion Supplement

Production of Myeloid Cells from Human CB-Derived CD34+ Cells Cultured in StemSpan™ SFEM II Containing Myeloid Expansion Supplement

Number of total nucleated cells (TNCs) produced per input human CB-derived CD34+ cell from 14 different donors and percentage of cells positive for myeloid markers CD13, CD14 and CD15 produced after 14 days of culture in SFEM II containing Myeloid Expansion Supplement. Best expansion was obtained in SFEM II, but myeloid cell expansion may also be obtained in SFEM and ACF media.
*95% confidence limits (CL), the range within which 95% of the results will typically fall.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
StemSpan™ Myeloid Expansion Supplement (100X)
Catalog #
02693
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
StemSpan™ Myeloid Expansion Supplement (100X)
Catalog #
02693
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

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Species Human
Contains • Recombinant human stem cell factor (SCF) • Recombinant human thrombopoietin (TPO) • Recombinant human granulocyte colony-stimulating factor (G-CSF) • Recombinant human granulocyte/macrophage colony-stimulating factor (GM-CSF)
Formulation Category Serum-Free
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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