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FreSR™- S

用于将胚胎干细胞和诱导多能干细胞以单细胞形式冻存的无动物成分冻存液
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产品号 #05859_C

用于将胚胎干细胞和诱导多能干细胞以单细胞形式冻存的无动物成分冻存液

产品优势

  • 成分明确、无血清、无动物成分的冻存液，用于将ESC/iPSC以单细胞形式冻存
  • ESC/iPSC复苏后可迅速恢复细胞集落
  • 可重复的高回收率
  • 针对在TeSR™维持培养基中培养的ESC/iPSC的冻存进行了优化
  • 保持ESC/iPSC的多能性和扩增能力
  • 便捷、即用型
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总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
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总览

FreSR™-S是一种成分确定、无血清、无动物成分的冻存液，用于将人多能干细胞（hPSCs）以单细胞形式进行冻存。这款完全即用型冻存液推荐用于使用mTeSR™ 1、mTeSR™ Plus、TeSR™ 2或TeSR™-E8™中培养的hPSCs。冻存的hPSCs应在-135°C（液氮）或更低的温度下储存。

细胞类型
多能干细胞
 
种属
人
 
应用
冻存
 
品牌
mFreSR，TeSR
 
研究领域
干细胞生物学
 
制剂类别
Animal Component-Free，无血清
 

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Data Figures

High Viability and Recovery of Cells Stored in FreSR™-S

Figure 1. High Viability and Recovery of Cells Stored in FreSR™-S

hPSCs cryopreserved as single cells using FreSR™-S have (A) higher post-thaw recovery (number of cells recovered / number of cells frozen) and (B) maintain higher viability (number of live cells / total number of cells) compared to competitor medium. All data values are plotted as percentages, (n=18, p < 0.0001 for each).

More Vials Frozen and Banked With FreSR™-S

Figure 2. More Vials Frozen and Banked With FreSR™-S

hPSCs are cryopreserved in FreSR™-S at lower cell density compared to traditional methods. Graph indicates the number of vials cryopreserved for each well of a 6 well plate that is harvested.
Note: 1 vial is typically thawed and seeded directly into 1 well of a six well plate. Cells should be cultured as aggregates after thawing.

hPSCs Frozen and Thawed as Single Cells with FreSR™-S Display a Normal Karyotype

Figure 3. hPSCs Frozen and Thawed as Single Cells with FreSR™-S Display a Normal Karyotype

Karyograms of (A-B) WLS-4D1 hiPS cells and (C-D) H1 hES cells that were frozen and thawed as single cells using FreSR™-S. (A, C) Thawed cells were seeded into culture containing TeSR™-E8™ medium and 10 µM Y-27632 and maintained as aggregates for five passages. (B,D) hPSCs were also subjected to a second freeze-thaw cycle as single cells with FreSR™-S and cultured for five passages as aggregates prior to collection of karyotype data.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
FreSR™-S
Catalog #
05859
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
FreSR™-S
Catalog #
05859
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (10)

Cryopreservation Media for Stem Cell Research
Brochure
Cryopreservation Media for Stem Cell Research
Maximize Your Pluripotential with the TeSR™ Family of hPSC Culture Media
Brochure
Maximize Your Pluripotential with the TeSR™ Family of hPSC Culture Media
hPSC-Derived Cell Therapy Workflow
Brochure
hPSC-Derived Cell Therapy Workflow
Products for Human Pluripotent Stem Cells
Brochure
Products for Human Pluripotent Stem Cells
hPSC Differentiation: Tools for Pluripotent Stem Cell-Derived Research
Brochure
hPSC Differentiation: Tools for Pluripotent Stem Cell-Derived Research
Directed Differentiation of Pluripotent Stem Cells
Wallchart
Directed Differentiation of Pluripotent Stem Cells
Pluripotent Stem Cell Biology
Wallchart
Pluripotent Stem Cell Biology
Stem Cell States: Naive to Primed Pluripotency
Wallchart
Stem Cell States: Naive to Primed Pluripotency
Improving Reproducibility of Your hPSC Research by Generating a High-Quality Cell Bank
21:53
Webinar
Improving Reproducibility of Your hPSC Research by Generating a High-Quality Cell Bank
Pluripotent Stem Cell Course
On-Demand Training
Pluripotent Stem Cell Course

Publications (1)

Recurrent gain of chromosomes 17q and 12 in cultured human embryonic stem cells. Draper JS et al. Nature biotechnology 2004 JAN

Abstract

We have observed karyotypic changes involving the gain of chromosome 17q in three independent human embryonic stem (hES) cell lines on five independent occasions. A gain of chromosome 12 was seen occasionally. This implies that increased dosage of chromosome 17q and 12 gene(s) provides a selective advantage for the propagation of undifferentiated hES cells. These observations are instructive for the future application of hES cells in transplantation therapies in which the use of aneuploid cells could be detrimental.
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Species Human
Formulation Category Animal Component-Free, Serum-Free
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