产品号 #03818_C
半固体甲基纤维素培养基，用于细胞的选择和克隆
概述
ClonaCell™FLEX是一种基础甲基纤维素培养基，需要添加用户自己液体培养基的2X浓缩物来创建定制的半固体克隆培养基。这允许用户将现有的液体克隆方法调整为定制的半固体克隆方法。ClonaCell™FLEX推荐用于选择和克隆适应悬液的哺乳动物细胞，包括CHO细胞和杂交瘤。培养基是化学定义的，不含动物成分，不含蛋白质。它不含l -谷氨酰胺、选择剂或酚红。ClonaCell™FLEX与多种选择系统兼容，包括二氢叶酸还原酶（DHFR），谷氨酰胺合成酶（GS）和次黄嘌呤，氨基蝶呤，胸苷（HAT）。
半固态克隆的好处：
•单个克隆和它们的后代在半固体基质中保持定位，因为它们成长为不同的菌落。这可以防止在液体培养基中选择过程中，由于快速生长的细胞过度生长而导致稀有高产克隆的损失，并有助于分离出具有广泛生长速率和生产力范围的多种克隆，以供下游筛选。
•从半固体培养基中获得的菌落具有很高的单克隆可能性，与有限稀释克隆相比，可以在更短的时间内使用更少的资源生成克隆细胞系。
•菌落可以通过人工或机器人方法轻松地从半固体培养基中挑选出来，并分散到液体培养基中进行筛选和扩增。
Contains
• Methylcellulose
• Water
Subtype
Semi-Solid Media, Specialized Media
Cell Type
CHO Cells, HEK-293, Hybridomas, Other
Species
Mouse, Other
Application
Cell Culture, Semi-Solid Cloning
Brand
ClonaCell
Area of Interest
Antibody Development, Cell Line Development, Drug Discovery and Toxicity Testing, Hybridoma Generation
Formulation Category
Animal Component-Free, Chemically Defined, Protein-Free, Serum-Free
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Resources and Publications
Educational Materials (11)
Frequently Asked Questions
更多信息
| Species | Mouse, Other |
|---|---|
| Contains | • Methylcellulose • Water |
| Formulation Category | Animal Component-Free, Chemically Defined, Protein-Free, Serum-Free |
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PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
