The CRISPR-Cas9 genome editing system enables high-efficiency genome editing to generate functional gene-edited cells. Bring this system and its best practices to your lab by registering for this three-day course consisting of live-streamed, interactive lectures and laboratory demonstrations. Guided by STEMCELL Technologies’ in-house pluripotent stem cell experts, you will gain the skills to successfully derive and maintain CRISPR-Cas9 genome-edited human pluripotent stem cells (hPSCs).
Learning Outcomes:
- Describe the evolution of genome editing techniques and hPSC culture
- Know the advantages and mechanism of CRISPR-Cas9
- Understand the potential and challenges associated with CRISPR-Cas9 in hPSCs
- Be familiar with a typical hPSC genome editing workflow (knock out and insertion of point mutations by knock-in using an ssODN template)
- Derivation of clonal lines
- Distinguish CRISPR-Cas9 guide RNA (gRNA) formats
- Understand and apply the principles of gRNA design to design a gRNA sequence and knock-in DNA donor template for point mutations
- Identify contributing factors to off-target effects of CRISPR-Cas9
- Understand considerations for knockout and knock-in experiments
- Discern the benefits and drawbacks of different methods of determining genome editing efficiencies to identify the most suitable method for your research objectives/applications
- Understand the basics of hPSC cell quality and considerations for hPSC research
As part of the course, you will receive:
- An electronic copy of the course book
- Certificate of course completion from STEMCELL Technologies
- Post-training support from the trainers and STEMCELL’s Scientific Support team
Space is limited, please apply early for the best chance of securing a seat for your desired date. Registration closes one week before the course date.
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Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages
Workflow Stages for Human Pluripotent Stem Cell Research
Workflow Stages for Genome Editing
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