The EasySep™ HLA Chimerism Whole Blood B Cell Positive Selection Kit is designed to isolate B cells from fresh whole blood or buffy coat by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD19, CD20 and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD19 antigen is present on mature B cells and B cell progenitors, but is lost upon maturation to plasma cells. The antigen CD20 is expressed on all stages of B cell development except on pro B cells or plasma cells.
This product replaces the EasySep™ HLA Whole Blood B Cell Positive Selection Kit (Catalog #18184HLA) for even faster cell isolations.
This product replaces the EasySep™ HLA Whole Blood B Cell Positive Selection Kit (Catalog #18184HLA) for even faster cell isolations.
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Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
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Educational Materials (13)
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
