EasySep™ Direct HLA Crossmatch B Cell Isolation Kit

Immunomagnetic negative selection from whole blood, spleen, or lymph nodes kit

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  • > 99.9% RBC depletion without the need for density gradient centrifugation, sedimentation, or lysis

  • Up to 99.8% purity of isolated cells

  • Fast, easy-to-use, and column-free

  • Isolated cells are untouched

  • The EasySep™ Direct HLA Crossmatch B Cell Isolation Kit can be used to isolate highly purified B cells directly from human whole blood, spleen, or lymph nodes by immunomagnetic negative selection. This cell isolation kit targets non-B cells for removal with antibodies recognizing specific surface markers. Unwanted cells are labeled with antibodies and EasySep™ Direct RapidSpheres™, and separated using an EasySep™ magnet. Desired cells are simply collected into a new tube and and are immediately available for downstream applications such as flow cytometry crossmatch.

    Please Note: If you are located in Canada, the EU, UK, or Australia, this product is not available in your region. To see the available equivalent product, please visit the EasySep™ Direct HLA B Cell Isolation Kit (Catalog #89684) product page.

    Data Figures

    Starting with human whole blood from normal healthy donors, the B cell (CD19+) content of the non-lysed final isolated fraction typically ranges from 89 - 99.8% (gated on CD45). In the above example, the B cell (CD19+) content of the lysed whole blood start sample and non-lysed final isolated fraction is 12.3% and 98.4% (gated on CD45), respectively.

    Protocols and Documentation

    Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

    Document Type
    Product Name
    Catalog #
    Lot #
    Language
    Catalog #
    19684
    Lot #
    All
    Language
    English
    Catalog #
    19684RF
    Lot #
    All
    Language
    English
    Document Type
    Safety Data Sheet
    Catalog #
    19684
    Lot #
    All
    Language
    English
    Document Type
    Safety Data Sheet
    Catalog #
    19684RF
    Lot #
    All
    Language
    English

    Applications

    This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

    PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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