Use Dispase, Animal Component-Free (ACF) for tissue dissociation and cell isolation procedures. This neutral amino-endoprotease cleaves the N-terminal peptide bond of non-polar amino acid residues, and is obtained from cultures free of animal-derived materials. The mild proteolytic activity of Dispase makes it especially useful for the isolation and passaging of primary cells, and facilitates maintenace of membrane integrity. Dispase is commonly used in combination with other proteases such as collagenase in cell isolation and tissue dissociation for many cell types: neural tissue (Dietrich et al.), kidney (Presnell et al.), epithelial tissue (Smoot et al.), endothelial tissue (Müller et al.), lung (Barkauskas et al.), colon (Roig et al.), and stem cells (Thomson et al.; Salmon et al.).
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Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Dispase, ACF
Catalog #
100-0396, 07446
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Dispase, ACF
Catalog #
100-0396, 07446
Lot #
All
Language
English
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages
Workflow Stages for Human Pluripotent Stem Cell Research
Workflow Stages for Mammary Epithelial Cell Research
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