Use DisCoZyme 2, Animal Component-Free (ACF) for tissue dissociation in applications where prevention of the introduction of potential animal-derived pathogens is necessary. This collagenase and dispase blend is sourced from clostridium histolyticum and bacillus polymyxa, respectively, and contains a minimum of 250 collagenase units/mg and 2000 caseinase units/mg of dry weight. Digest native collagen fibrils commonly found in connective tissues while the neutral amino-endoprotease, dispase, cleaves the N-terminal peptide bond of non-polar amino acid residues. The mild proteolytic activity of dispase allows it to maintain membrane integrity while isolating and passaging of primary cells. DisCoZyme 2, ACF is designed to contain high levels of collagenase and caseinase activity and has been used for the digestion of lung (Fujino et al.; Driscoll et al.) and neural tissues (Panchision et al.). Use DisCoZyme 1, ACF (Catalog #07449), if lower levels of caseinase activity are desired.
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Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
DisCoZyme 2, ACF
Catalog #
07450
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
DisCoZyme 2, ACF
Catalog #
07450
Lot #
All
Language
English
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages
Workflow Stages for Mammary Epithelial Cell Research
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