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STEMdiff™ 感觉神经元分化试剂盒

用于从人 iPS 细胞衍生的神经嵴细胞生成感觉神经元前体的分化试剂盒
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产品号 #100-0341_C

用于从人 iPS 细胞衍生的神经嵴细胞生成感觉神经元前体的分化试剂盒

产品优势

  • 通过消除昂贵的背根神经节外植体工作流程来减少动物使用
  • 使用 NCC 中间体和 STEMdiff™ 神经嵴分化试剂盒，从多个人类诱导多能干细胞 (iPS) 系中分化出感觉神经元
  • 通过简单易用的培养基格式简化 PSC 衍生的感觉神经元生成
  • 通过集成 BrainPhys™ 培养基支持神经元活动和成熟，获得生理相关结果

产品组分包括

  • STEMdiff™ 感觉神经元分化基础培养基，100 mL
  • STEMdiff™ 感觉神经元分化补充剂，1 mL
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Need a high-quality cell source? Use the hiPSC SCTi003-A (female) or SCTi004-A (male) control lines, manufactured with mTeSR™ Plus.
总览
实验数据
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应用领域
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总览

用这个易于使用的培养系统在培养皿中产生功能性的、人类特异性的感觉神经元。使用STEMdiff™神经嵴分化试剂盒获得多能干细胞（PSC）来源的神经嵴细胞（NCCs）后，使用无血清STEMdiff™感觉神经元分化试剂盒生成感觉神经元前体。STEMdiff™感觉神经元成熟试剂盒可用于维持和成熟感觉神经元前体。得到的细胞brn3a阳性，超过70%的tuj1阳性。以BrainPhys™提供的生理葡萄糖和渗透压条件为基础培养基，神经元表现出对感觉配体和温度变化的反应活性。生成的功能性人类感觉神经元群可用于药物发现和疼痛研究应用。

亚型
专用培养基
 
细胞类型
神经细胞，PSC衍生，神经元
 
种属
人
 
应用
细胞培养，分化
 
品牌
BrainPhys，STEMdiff
 
研究领域
疾病建模，药物发现和毒理检测，神经科学
 
制剂类别
无血清
 

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Data Figures

Experimental Protocol for STEMdiff™ Sensory Neuron Differentiation and Maturation Kits

Figure 1. Schematic for the STEMdiff™ Sensory Neuron Culture System Protocol

Sensory neuron precursors can be generated in 6 days from hPSC-derived neural crest cells. For the generation of neural crest cells, see documentation for STEMdiff™ Neural Crest Differentiation Kit (Catalog #08610). For the maturation of sensory neuron precursors to sensory neurons, see the PIS.

Figure 2. STEMdiff™ Sensory Neuron Kits Promote Differentiation Across Multiple Embryonic Stem and Induced Pluripotent Stem Cell Lines

NCCs generated from hPSCs in mTeSR™ Plus using the STEMdiff™ Neural Crest Differentiation Kit were differentiated and matured to sensory neurons using the STEMdiff™ Sensory Neuron Differentiation and Maturation Kits. (A) Sensory neurons were generated after hPSC-derived NCCs were cultured with the STEMdiff™ Sensory Neuron Differentiation Kit for 6 days and then the STEMdiff™ Sensory Neuron Maturation Kit for 6 days. The resulting cultures contain a population of cells expressing sensory neuron markers peripherin (green) and BRN3A (red) along with (B) neuronal marker class III β-tubulin (TUJ1, red). (C) Midbrain neuron controls generated with STEMdiff™ Midbrain Neuron Differentiation and Maturation Kits do not have detectable peripherin (green) or BRN3A (red) expression, although they express (D) neuronal marker class III β-tubulin (TUJ1, red). Nuclei are labeled with DAPI (blue). Human ES and iPS cell lines were maintained in either mTeSR™1, TeSR™-E8™, or mTeSR™ Plus and differentiated with STEMdiff™ Neural Crest Differentiation Kit, followed by STEMdiff™ Sensory Neuron Differentiation and Maturation Kits. The percentage expression of (E) BRN3A+ and (F) TUJ1+ cells in the resulting cultures was quantified. This differentiation generated BRN3A+ sensory neurons (25.3% ± 6.9%, mean ± SEM; n=7 cell lines, 3 - 23 replicates per condition) that expressed neuronal marker class III β-tubulin (TUJ1; 90.3% ± 4.1%, mean ± SEM; n=4 cell lines, 3 - 12 replicates per condition). Numbers are % positive over total DAPI in a tiled image. NCCs = neural crest cells; hPSCs = human pluripotent stem cells; ES = embryonic stem; iPS = induced pluripotent stem

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
STEMdiff™ Sensory Neuron Differentiation Kit
Catalog #
100-0341
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
STEMdiff™ Sensory Neuron Differentiation Kit
Catalog #
100-0341
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
STEMdiff™ Sensory Neuron Differentiation Kit
Catalog #
100-0341
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

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Species Human
Formulation Category Serum-Free
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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    ISO 14001 环境管理体系认证

Scientists Helping Scientists™

ISO 13485 和 ISO 9001 质量管理体系认证

ISO 14001 环境管理体系认证

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