The RTK4530 antibody (IgG2b, kappa) is suitable for use as an isotype-matched control antibody in several applications to estimate the degree of non-specific binding by an antigen-specific antibody. Ideally, the isotype control should have the same subclass of heavy chain (IgA, IgD, IgE, IgG, or IgM) and light chain (kappa or lambda) as the specific antibody being used. If a conjugated antibody is used, an isotype control conjugated to the same molecule (e.g. fluorochrome) should be chosen. The use of an appropriate isotype control helps confirm the specificity of the antigen-specific antibody and indicates non-specific binding that may result from binding to Fc receptors or other cell components.
This antibody clone has been verified for use as an isotype control antibody for assessing non-specific binding to cells in applications such as flow cytometry and immunofluorescence microscopy applications (surface and intracellular staining).
This antibody clone has been verified for use as an isotype control antibody for assessing non-specific binding to cells in applications such as flow cytometry and immunofluorescence microscopy applications (surface and intracellular staining).
Data Figures
Figure 1. Data for Unconjugated
Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, followed by a mouse anti-rat IgG2b antibody, FITC (solid line histogram). Filled histogram shows labeling with a rat IgG2b, kappa positive control antibody (Anti-Mouse CD45 Antibody, Clone 30-F11, Catalog #60030) followed by a mouse anti-rat IgG2b antibody, FITC.
Figure 2. Data for Alexa Fluor® 488-Conjugated
Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, Alexa Fluor® 488 (solid line histogram). Filled histogram shows labeling with a rat IgG2b, kappa positive control antibody (Anti-Mouse CD45 Antibody, Clone 30-F11, Alexa Fluor® 488, Catalog #60030AD).
Figure 3. Data for APC-Conjugated
Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, APC (solid line histogram). Filled histogram shows labeling with a rat IgG2b, kappa positive control antibody (Anti-Mouse CD45 Antibody, Clone 30-F11, APC, Catalog #60030AZ).
Figure 4. Data for Biotin-Conjugated
Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, Biotin followed by streptavidin (SAV) APC (solid line histogram). Filled histogram shows labeling with a rat IgG2b, kappa positive control antibody (Anti-Mouse CD45 Antibody, Clone 30-F11, Biotin, Catalog #60030BT) followed by SAV APC.
Figure 5. Data for FITC-Conjugated
Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, FITC (solid line histogram). Filled histogram shows labeling with a rat IgG2b, kappa positive control antibody (Anti-Mouse CD45 Antibody, Clone 30-F11, FITC, Catalog #60030FI).
Figure 6. Data for PE-Conjugated
Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, PE (solid line histogram). Filled histogram shows labeling with a rat IgG2b, kappa positive control antibody (Anti-Mouse CD45 Antibody, Clone 30-F11, PE, Catalog #60030PE).
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Catalog #
60077FI.2, 60077FI, 60077FI.3, 60077FI.1
Lot #
All
Language
English
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
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Workflow Stages
Resources and Publications
Educational Materials (2)
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Legal Statement: Alexa Fluor is a registered trademark of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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