The MC-631 antibody reacts with a galactosylgloboside epitope on the stage-specific embryonic antigen-3 (SSEA-3), which is expressed on the surface of human embryonal carcinoma (EC), embryonic germ (EG), undifferentiated embryonic stem (ES), and induced pluripotent stem (iPS) cells, as well as rhesus monkey ES cell lines. No immunoreactivity is evident with undifferentiated mouse EC, EG and ES cells. Expression of SSEA-3 is down regulated following differentiation of ES and EC cells. In contrast, the differentiation of mouse ES and EC cells may be accompanied by an increase in SSEA-3 expression.
This antibody clone has been verified for labeling human ES and iPS cells grown in TeSR™-E8™ (Catalog #05940), mTeSR™1 (Catalog #85850) and TeSR™2 (Catalog #05860) and has been verified for purity assessments of cells isolated with EasySep™ kits, including EasySep™ Human ES/iPS Cell TRA-1-60 Positive Selection Kit (Catalog #18166).
This antibody clone has been verified for labeling human ES and iPS cells grown in TeSR™-E8™ (Catalog #05940), mTeSR™1 (Catalog #85850) and TeSR™2 (Catalog #05860) and has been verified for purity assessments of cells isolated with EasySep™ kits, including EasySep™ Human ES/iPS Cell TRA-1-60 Positive Selection Kit (Catalog #18166).
Data Figures
Figure 1. Data for Unconjugated
(A) Flow cytometry analysis of human ES cells (filled histogram) or fibroblasts (negative control; dashed line histogram) labeled with Anti-Mouse SSEA-3 Antibody, Clone MC-631, followed by Goat Anti-Rat IgM (Heavy Chain) Antibody, Polyclonal, APC (Catalog #60140AZ). Labeling of human ES cells with a rat IgM isotype control antibody, followed by Goat Anti-Rat IgM (Heavy Chain) Antibody, Polyclonal, APC is shown (solid line histogram). (B) Human ES cells were cultured in mTeSR™1 on Corning® Matrigel®-coated glass slides, then fixed and stained with Anti-Mouse SSEA-3 Antibody, Clone MC-631, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (Catalog #60138FI). Inset shows cells labeled with a rat IgM, kappa isotype control antibody, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC.
Figure 2. Data for Alexa Fluor® 488-Conjugated
(A) Flow cytometry analysis of human ES cells (filled histogram) or HT1080 fibrosarcoma cells (negative control; dashed line histogram) labeled with AntiMouse SSEA-3 Antibody, Clone MC-631, Alexa Fluor® 488. Labeling of human ES cells with a rat IgM, kappa Alexa Fluor® 488 isotype control antibody is shown (solid line histogram). (B) Human ES cells were cultured in mTeSR™1 on Corning® Matrigel®-coated glass slides, then fixed and stained with Anti-Mouse SSEA-3 Antibody, Clone MC-631, Alexa Fluor® 488. Inset shows cells labeled with a rat IgM, kappa Alexa Fluor® 488 isotype control antibody. (C) Flow cytometry analysis of human iPS cells labeled with Anti-Mouse SSEA-3 Antibody, Clone MC-631, Alexa Fluor® 488 (filled histogram) or a rat IgM, kappa Alexa Fluor® 488 isotype control antibody (solid line histogram).
Figure 3. Data for PE-Conjugated
(A) Flow cytometry analysis of human ES cells (filled histogram) or HT1080 fibrosarcoma cells (negative control; dashed line histogram) labeled with AntiMouse SSEA-3 Antibody, Clone MC-631, PE. Labeling of human ES cells with a rat IgM, kappa PE isotype control antibody is shown (solid line histogram). (B) Human ES cells were cultured in mTeSR™1 on Corning® Matrigel®-coated glass slides, then fixed and stained with Anti-Mouse SSEA-3 Antibody, Clone MC-631, PE. Inset shows cells labeled with a rat IgM, kappa PE isotype control antibody. (C) Flow cytometry analysis of human iPS cells labeled with Anti-Mouse SSEA-3 Antibody, Clone MC-631, PE (filled histogram) or a rat IgM, kappa PE isotype control antibody (solid line histogram).
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Anti-Mouse SSEA-3 Antibody, Clone MC-631, PE
Catalog #
60061PE
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse SSEA-3 Antibody, Clone MC-631
Catalog #
60061.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse SSEA-3 Antibody, Clone MC-631, PE
Catalog #
60061PE
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse SSEA-3 Antibody, Clone MC-631
Catalog #
60061.1
Lot #
All
Language
English
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages
Workflow Stages for Mouse Pluripotent Stem Cell Research
Workflow Stages for PSC Quality Control
Resources and Publications
Educational Materials (3)
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Legal Statement: Alexa Fluor is a registered trademark of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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