Efficiently expand human pluripotent stem cell (hPSC)-derived endothelial cells using xeno-free STEMdiff™ Endothelial Expansion Culture Kit (Catalog #100-1218). This kit includes xeno-free STEMdiff™ Endothelial Expansion Basal Medium and 5X Supplement, and Animal Component-Free Cell Attachment Substrate, and is is optimized for the expansion of hPSC-derived endothelial cells and for the maintenance of endothelial marker expression. After 7 days of mesoderm and endothelial induction, the resulting cells can be expanded for 5 - 6 passages to generate large numbers of highly pure, mature endothelial cells that typically reach ≥ 99% CD144+CD31+ by passage 2 and maintain this phenotype until passage 5.
This kit is compatible with hPSCs maintained in mTeSR™1 (Catalog #85850), mTeSR™ Plus (Catalog #100-0276), or TeSR™-E8™ (Catalog #05990) on Corning® Matrigel®. The components of STEMdiff™ Endothelial Expansion Culture Kit are included in STEMdiff™ Endothelial Differentiation Kit (Catalog #08005). For passaging, Animal Component-Free Cell Dissociation Kit (Catalog #05426) is required.
NOTE: Heparin Solution (Catalog #07980) is required for preparation of complete STEMdiff™ Endothelial Expansion Medium.
CollPlant is the manufacturer of the rhCollagen component of the cell attachment substrate.
This product may be used for research purposes only. Please contact STEMCELL for any clinical or commercial application of the Product.
This kit is compatible with hPSCs maintained in mTeSR™1 (Catalog #85850), mTeSR™ Plus (Catalog #100-0276), or TeSR™-E8™ (Catalog #05990) on Corning® Matrigel®. The components of STEMdiff™ Endothelial Expansion Culture Kit are included in STEMdiff™ Endothelial Differentiation Kit (Catalog #08005). For passaging, Animal Component-Free Cell Dissociation Kit (Catalog #05426) is required.
NOTE: Heparin Solution (Catalog #07980) is required for preparation of complete STEMdiff™ Endothelial Expansion Medium.
CollPlant is the manufacturer of the rhCollagen component of the cell attachment substrate.
This product may be used for research purposes only. Please contact STEMCELL for any clinical or commercial application of the Product.
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Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
STEMdiff™ Endothelial Expansion Culture Kit
Catalog #
100-1218
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
STEMdiff™ Endothelial Expansion Culture Kit
Catalog #
100-1218
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
STEMdiff™ Endothelial Expansion Culture Kit
Catalog #
100-1218
Lot #
All
Language
English
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages
Workflow Stages for Human Pluripotent Stem Cell Research
Workflow Stages for hPSC-Derived Mesoderm Cell Research
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