STEMdiff™ Blood Vessel Organoid Kit
Media and supplements for differentiation of human pluripotent stem cells to blood vessel organoids
Generate physiologically relevant blood vessel organoids—easily and with high yield—for your tissue repair and regeneration studies or to model vascular disease in vitro by using STEMdiff™ Blood Vessel Organoid Kit.
Use STEMdiff™ Blood Vessel Organoid Kit for efficient and reproducible generation of blood vessel organoids from human pluripotent stem cells (hPSCs), with the option to scale up for high-throughput screening in a 96-well format. Compared to homemade organoid culture media formulations, organoids generated with this easy-to-use kit develop extensive and physiologically relevant vascular networks and strong endothelial cell-pericyte interactions. Blood vessel organoids generated using this kit have CD31+/CD34+/CD144+/KDR+ endothelial cells and PDGFR-β+/CD146+/SMA+/NG-2+ pericytes.The simple protocol also eliminates the need for serum screening and labor-intensive protocol optimization, so you can spend more time on what matters—your experiments.
Use STEMdiff™ Blood Vessel Organoid Kit with mTeSR™ Plus or mTeSR™1 culture media for an easy transition from hPSCs to organoids. For your convenience, STEMdiff™ Blood Vessel Organoid Maturation Medium, required for the maturation of hPSC-derived blood vessel organoids, is available either as a part of the kit or for individual sale. To differentiate hPSCs to endothelial cells, use our STEMdiff™ Endothelial Differentiation Kit.
Use STEMdiff™ Blood Vessel Organoid Kit for efficient and reproducible generation of blood vessel organoids from human pluripotent stem cells (hPSCs), with the option to scale up for high-throughput screening in a 96-well format. Compared to homemade organoid culture media formulations, organoids generated with this easy-to-use kit develop extensive and physiologically relevant vascular networks and strong endothelial cell-pericyte interactions. Blood vessel organoids generated using this kit have CD31+/CD34+/CD144+/KDR+ endothelial cells and PDGFR-β+/CD146+/SMA+/NG-2+ pericytes.The simple protocol also eliminates the need for serum screening and labor-intensive protocol optimization, so you can spend more time on what matters—your experiments.
Use STEMdiff™ Blood Vessel Organoid Kit with mTeSR™ Plus or mTeSR™1 culture media for an easy transition from hPSCs to organoids. For your convenience, STEMdiff™ Blood Vessel Organoid Maturation Medium, required for the maturation of hPSC-derived blood vessel organoids, is available either as a part of the kit or for individual sale. To differentiate hPSCs to endothelial cells, use our STEMdiff™ Endothelial Differentiation Kit.
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Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
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Resources and Publications
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Legal Statement: This product was developed under a license to intellectual property owned by the Institute of Molecular Biotechnology (IMBA) of the Austrian Academy of Sciences. This product is sold for research use only (whether the buyer is an academic or for-profit entity) under a non-transferable, limited-use license. Purchase of this product does not include the right to sell, use or otherwise transfer this product for commercial purposes (i.e., any activity undertaken for consideration, such as use of this product for manufacturing, or resale of this product or any materials made using this product, or use of this product or any materials made using this product to provide services or, in collaboration with, a for-profit entity, for purposes other than research applications (i.e., drug development activities). Purchasers wishing to use the product for commercial purposes should contact IMBA at technologvtransfer@imba.oeaw.ac.at. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
