The XMG1.2 antibody reacts with mouse interferon-gamma (IFN-γ), a glycosylated type II interferon comprising a homodimer of ~20 kDa subunits in its active form. IFN-γ is produced primarily by activated T lymphocytes and natural killer (NK) cells in response to antigens, including alloantigens, mitogens, and enterotoxins. IFN-γ binds with high affinity to the α subunit of the IFN-γ receptor, resulting in the ligand-induced assembly of a tetrameric α2β2 receptor complex capable of mediating the cellular response to the cytokine through activation of the JAK-STAT pathway. The IFN-γ receptor is expressed on the surface of all nucleated cells. IFN-γ has important functions in a wide range of biological processes, including macrophage activation, mediating the immune response to viruses and bacteria, and modulating cellular proliferation and apoptosis. The XMG1.2 antibody is suitable for detection of intracellular IFN-γ by methods such as flow cytometry, when paired with an appropriate secondary antibody. The XMG1.2 antibody is also widely used in various assays to neutralize the functional activity of IFN-γ.
Data Figures
Figure 1. Data for Anti-Mouse IFN-gamma Antibody, Clone XMG1.2, APC
Flow cytometry analysis of (A) unstimulated C57BL/6 mouse splenocytes labeled with Anti-Mouse IFN-gamma Antibody, Clone XMG1.2, APC, and anti-mouse CD3e antibody, clone 145-2C11, PE and (B) PMA/ionomycin-stimulated C57BL/6 mouse splenocytes labeled with Anti-Mouse IFN-gamma Antibody, Clone XMG1.2, APC, and anti-mouse CD3e antibody, clone 145-2C11, PE.
Figure 2. Data for Anti-Mouse IFN-gamma Antibody, Clone XMG1.2, Biotin
Flow cytometry analysis of PMA/ionomycin-stimulated C57BL/6 mouse splenocytes labeled with Anti-Mouse IFN-γ Antibody, Clone XMG1.2, Biotin, followed by streptavidin (SAV) PE.
Figure 3. Data for Anti-Mouse IFN-gamma Antibody, Clone XMG1.2, FITC
Flow cytometry analysis of PMA/ionomycin-stimulated C57BL/6 mouse splenocytes labeled with Anti-Mouse IFN-gamma Antibody, Clone XMG1.2, FITC.
Figure 4. Data for Anti-Mouse IFN-gamma Antibody, Clone XMG1.2, PE
Flow cytometry analysis of (A) unstimulated C57BL/6 mouse splenocytes labeled with Anti-Mouse IFN-gamma Antibody, Clone XMG1.2, PE, and anti-mouse CD3e antibody, clone 145-2C11, APC and (B) PMA/ionomycin-stimulated C57BL/6 mouse splenocytes labeled with Anti-Mouse IFN-gamma Antibody, Clone XMG1.2, PE, and anti-mouse CD3e antibody, clone 145-2C11, APC.
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Anti-Mouse IFN-gamma Antibody, Clone XMG1.2, APC
Catalog #
100-0278, 100-0277
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse IFN-gamma Antibody, Clone XMG1.2, APC
Catalog #
100-0278, 100-0277
Lot #
All
Language
English
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