Simple monolayer protocol, compatible with STEMdiff™ cardiomyocyte workflow
Establishment and efficient generation of up to 50 million atrial cardiomyocytes per kit
Cardiomyocytes generated with this serum-free kit express high levels of atrial-specific genes and demonstrate dominant atrial-like phenotypes including faster beat rate and decreased field potential duration when compared to those generated using the STEMdiff™ Cardiomyocyte Differentiation Kit
Maximum reproducibility with minimal variability across multiple hPSC lines
STEMdiff™ Atrial Cardiomyocyte Differentiation Kit is optimized for the differentiation and maintenance of human embryonic stem and induced pluripotent stem cells into atrial cardiomyocytes (cardiac troponin T-positive; cTnT+). This serum-free kit can be used to generate atrial cardiomyocytes derived from a clump culture of human pluripotent stem cells (hPSCs) maintained in mTeSR™1 (Catalog #85850), mTeSR™ Plus (Catalog #100-0276), or TeSR™-E8™ (Catalog #05990). With greater than 80% yield purity, an average of 1 x 10^6 cTnT+ cells can be harvested from a single well of a 12-well plate. STEMdiff™ Cardiomyocyte Maintenance Kit (Catalog #05020) comprises the maintenance basal medium and supplement and allows for long-term maintenance of hPSC-derived cardiomyocytes for one month or longer. These cardiomyocytes can be used in various downstream applications and analyses.
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Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
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