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STEMdiff™ Neural Crest Differentiation Kit

Cell culture kit for establishment of hPSC-derived neural crest cells
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产品优势


  • RAPID - Generates neural crest cells in an easy-to-use monolayer protocol in only six days

  • EFFICIENT - Greater than 70% purity of multipotent neural crest cells

  • MULTIPOTENT - Produces cells capable of downstream differentiation, including to peripheral neurons, chondrocytes, and osteoblasts

  • VERSATILE - Compatible with human ES and iPS cells maintained in mTeSR™1, mTeSR™ Plus, or TeSR™-E8™

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    概述
    实验数据
    产品说明书及文档
    应用领域
    相关材料与文献
    STEMdiff™ Neural Crest Differentiation Kit creates a serum-free medium for differentiation of human embryonic stem (ES) cells and induced pluripotent stem (iPS) cells to neural crest cells. These neural crest cells, which are characterized by neural crest markers such as SOX10 and CD271, can be differentiated to several downstream derivatives including chondrocytes, osteoblasts, and peripheral neurons. This medium is compatible with human ES and iPS cells maintained in either mTeSR™1 (Catalog #85850), mTeSR™ Plus (Catalog #100-0276) or TeSR™-E8™ (Catalog #05990).

    Data Figures

    Figure 1.

    Neural crest cells are produced using STEMdiff™ Neural Crest Differentiation Kit after 6 days in culture. For information on continued passage of neural crest cells, contact us at techsupport@stemcell.com

    STEMdiff™ Neural Crest Differentiation Kit Generates a Highly Pure Population of NCCs with Minimal CNS-type Progenitors

    Figure 2. STEMdiff™ Neural Crest Differentiation Kit Generates a Highly Pure Population of NCCs with Minimal CNS-type Progenitors

    After 6 days in culture, neural crest cells (NCCs; SOX10+, red; CD271+, light blue) outnumber CNS-type progenitors (PAX6+, green). (A) Channel merge of cells fixed 2 days after being passaged on day 6. Individual immunofluorescence channels show (B) DAPI, (C) PAX6, (D) SOX10, and (E) CD271. Scale bar = 100 μm.

    NCCs Generated with the STEMdiff™ Neural Crest Differentiation Kit Are Multipotent

    Figure 3. NCCs Generated with the STEMdiff™ Neural Crest Differentiation Kit Are Multipotent

    NCCs (A) were cultured for 6 days and display typical morphology. (B) Culturing NCCs using established protocols generates peripheral neurons (Peripherin, green; BRN3a, red; DAPI, blue). (C) Passaging NCCs into MesenCult™-ACF Plus Medium and then into the MesenCult™-ACF Chondrogenic Differentiation Kit generates a chondrocyte pellet (Alcian Blue, Nuclear Fast Red) with deposition of cartilage around the cells. (D) Passaging NCCs into MesenCult™-ACF Plus Medium and then into the MesenCult™ Osteogenic Differentiation Kit (Human) generates an osteoblast culture with high levels of alkaline phosphatase-positive mineral deposition. Scale bar = 500 μm (A-C), 1 mm (D).

    Protocols and Documentation

    Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

    Document Type
    Product Name
    Catalog #
    Lot #
    Language
    Document Type
    Product Information Sheet
    Product Name
    STEMdiff™ Neural Crest Differentiation Kit
    Catalog #
    08610
    Lot #
    All
    Language
    English
    Document Type
    Safety Data Sheet
    Product Name
    STEMdiff™ Neural Crest Differentiation Kit
    Catalog #
    08610
    Lot #
    All
    Language
    English

    Applications

    This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

    Research Area
    Workflow Stages
    PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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