MyoCult™ Differentiation Kit (Human)

Medium for the differentiation of human skeletal muscle progenitor cells (myoblasts) into myotubes

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  • Supports differentiation of myogenic progenitor cells to multinucleated myotubes with strong expression of myosin heavy chain and myogenin markers

  • Rigorous raw material screening and quality control minimize lot-to-lot variability

  • Attachment substrate is included to support optimal adherence and enhanced myotube morphology

  • MyoCult™ Differentiation Kit (Human) includes MyoCult™ Differentiation Medium and Animal Component-Free (ACF) Cell Attachment Substrate. MyoCult™ Differentiation Medium is a serum-based medium specifically formulated for the in vitro differentiation of human skeletal muscle progenitor cells (myoblasts) into myotubes. MyoCult™ Differentiation Medium is designed for use with the ACF Cell Attachment Substrate, which has been specifically tested for its ability to support optimal adherence and myotube morphology. This medium is suitable for the differentiation of myogenic progenitor cells previously expanded in serum-containing or serum-free media (e.g. using the MyoCult™-SF Expansion Kit #05980).

    CollPlant is the manufacturer of the rhCollagen component of the cell attachment substrate.

    The Product may be used for research purposes only. Please contact STEMCELL for any clinical or commercial application of the Product.

    Data Figures

    Figure 1. MyoCult™ Differentiation Medium Induces Robust Differentiation of Myotubes from Human Myoblasts

    (A) Myogenic progenitor cells expanded in MyoCult expansion medium expressed MyoD (green) and desmin (red). (B) Robust differentiation of myoblasts (shown in (A)) to multinucleated myotubes expressing myosin heavy chain (MyHC+) was achieved following culture in MyoCult™ Differentiation Medium for as little as 5 days. (C) A fusion index showing approximately 75% of total nuclei were found within myotubes expressing MyHC.

    Protocols and Documentation

    Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

    Document Type
    Product Name
    Catalog #
    Lot #
    Language
    Catalog #
    05965
    Lot #
    All
    Language
    English
    Document Type
    Safety Data Sheet 1
    Catalog #
    05965
    Lot #
    All
    Language
    English
    Document Type
    Safety Data Sheet 2
    Catalog #
    05965
    Lot #
    All
    Language
    English
    Document Type
    Safety Data Sheet 3
    Catalog #
    05965
    Lot #
    All
    Language
    English

    Applications

    This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

    PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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