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STEMdiff™ Pancreatic Progenitor Kit

Serum-free medium for the differentiation of human ES and iPS cells to pancreatic progenitor cells
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产品优势


  • Generates functional pancreatic progenitor cells for transplantation into animal models or for further maturation into insulin-producing beta cells

  • Efficient and reproducible differentiation of multiple hPSC lines to pancreatic progenitor cells expressing PDX-1, NKX6.1, and SOX9

  • Serum-free medium

  • Compatible with multiple human ES and iPS cell lines maintained in mTeSR™1 or mTeSR™ Plus.

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    概述
    实验数据
    产品说明书及文档
    应用领域
    相关材料与文献
    STEMdiff™ Pancreatic Progenitor Kit is a serum-free medium that supports efficient and reproducible generation of pancreatic progenitor cells from human pluripotent stem cells (hPSCs). Cells are differentiated through four stages: 1) definitive endoderm, 2) primitive gut tube, 3) posterior foregut endoderm, and 4) pancreatic progenitor cells. The differentiated cells express key markers of pancreatic progenitor cells, including PDX-1, NKX6.1 and SOX9 and up-regulation of insulin and glucagon. The resulting pancreatic progenitor cells can be further differentiated to insulin-producing beta cells or other endocrine and exocrine pancreatic cell fates.

    STEMdiff™ Pancreatic Progenitor Kit has been optimized for differentiation of cells maintained in mTeSR™1 (Catalog #85850) or mTeSR™ Plus (Catalog #100-0276).

    Data Figures

    Pancreatic Differentiation of Progenitor Cells into Mature Endocrine and Exocrine Cells

    Figure 1. Pancreatic Progenitor Cells can Mature into Endocrine and Exocrine Cells

    A) Representative image showing pancreatic progenitor cells expressing PDX-1 (red) and NKX6.1 (green). Yellow staining indicates co‑expression of both markers in the majority of cells as is observed in the developing human pancreas.1 (B, C) Cells transplanted into mice can mature into endocrine and exocrine cells.
    B) shows endocrine clusters expressing synaptophysin (red) surrounded by ductal structures expressing CK-19 (green).
    C) Shows islet-like structures containing monohormonal cells that individually express insulin (red), glucagon (green) or somatostatin (blue). Data in (B, C) are from the laboratory of Dr. Timothy J. Kieffer (University of British Columbia, Vancouver, Canada).

    A. The STEMdiff™ Pancreatic Progenitor Kit Functions Efficiently Across Multiple hPSC Lines
    B. The STEMdiff™ Pancreatic Progenitor Kit Functions Efficiently Across Multiple hPSC Lines

    Figure 2. The STEMdiff™ Pancreatic Progenitor Kit Functions Efficiently Across Multiple hPSC Lines

    PDX-1 and NKX6.1 expression measured in pancreatic progenitor cells derived from four different hPSC lines (H1, H9, WLS-4D1 and WLS-1C) at the end of Stage 4. (A) Representative flow cytometry plots show PDX-1 and NKX6.1 co-expression in differentiated H9 cells. (B) Quantitative data for PDX-1/NKX6.1 co-expression in two human ES (H1 and H9) and two human iPS (WLS-4D1 and WLS-1C) cell lines (n = 5-10 per cell line). Data are plotted as individual points representing the mean of duplicates within a single experiment. The horizontal line represents the mean of all experiments, with error bars indicating the standard error of the mean (SEM). The average efficiency of pancreatic progenitor differentiation ranges from 61.5% to 77.7% depending on the cell line.

    Gene Expression Profile is Indicative of Transition to Pancreatic Progenitor Cells

    Figure 3. Gene Expression Profile is Indicative of Transition to Pancreatic Progenitor Cells

    Gene expression profile at the end of each stage of differentiation for key markers of pancreatic progenitor cells. Expression was normalized to 18S ribosomal RNA and TATA Binding Protein (TBP). Data are the mean ± SEM for 3 - 5 experiments. Expression pattern is consistent with published data.²
     
    1. Riedel M et al. (2012) Immunohistochemical characterization of cells co-producing insulin and glucagon in the developing human pancreas. Diabetologia 55(2): 372-81.
    2. Rezania A et al. (2014) Reversal of diabetes with insulin-producing cells derived in vitro from human pluripotent stem cells. Nat Biotechnol 32(11): 1121-33.

    Density plots and quantitative analysis showing PDX-1 and NKX6.1 expression in cells cultured in mTeSR™1 or mTeSR™ Plus, following 5 days of differentiation using the STEMdiff™ Pancreatic Progenitor Kit.

    Figure 4. Generation of Pancreatic Progenitors from hPSCs Maintained in mTeSR™ Plus

    (A) Representative density plots showing PDX-1 and NKX6.1 expression in cells cultured in mTeSR™1 (daily feeds) or mTeSR™ Plus (restricted feeds), following differentiation using the STEMdiff™ Pancreatic Progenitor Kit. (B) Quantitative analysis of pancreatic progenitor formation in multiple hPS (H9, STiPS-M001, WLS-1C) cell lines maintained with mTeSR™1 or mTeSR™ Plus as measured by co-expression of PDX-1 and NKX6.1. Data are expressed as the mean percentage of cells (± SEM) expressing both markers; n=3.

    Protocols and Documentation

    Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

    Document Type
    Product Name
    Catalog #
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    Document Type
    Product Information Sheet
    Product Name
    STEMdiff™ Pancreatic Progenitor Kit
    Catalog #
    05120
    Lot #
    All
    Language
    English
    Document Type
    Safety Data Sheet 1
    Product Name
    STEMdiff™ Pancreatic Progenitor Kit
    Catalog #
    05120
    Lot #
    All
    Language
    English
    Document Type
    Safety Data Sheet 2
    Product Name
    STEMdiff™ Pancreatic Progenitor Kit
    Catalog #
    05120
    Lot #
    All
    Language
    English
    Document Type
    Safety Data Sheet 3
    Product Name
    STEMdiff™ Pancreatic Progenitor Kit
    Catalog #
    05120
    Lot #
    All
    Language
    English
    Document Type
    Safety Data Sheet 4
    Product Name
    STEMdiff™ Pancreatic Progenitor Kit
    Catalog #
    05120
    Lot #
    All
    Language
    English
    Document Type
    Safety Data Sheet 5
    Product Name
    STEMdiff™ Pancreatic Progenitor Kit
    Catalog #
    05120
    Lot #
    All
    Language
    English
    Document Type
    Safety Data Sheet 6
    Product Name
    STEMdiff™ Pancreatic Progenitor Kit
    Catalog #
    05120
    Lot #
    All
    Language
    English
    Document Type
    Safety Data Sheet 7
    Product Name
    STEMdiff™ Pancreatic Progenitor Kit
    Catalog #
    05120
    Lot #
    All
    Language
    English
    Document Type
    Safety Data Sheet 8
    Product Name
    STEMdiff™ Pancreatic Progenitor Kit
    Catalog #
    05120
    Lot #
    All
    Language
    English

    Applications

    This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

    Research Area
    Workflow Stages

    Resources and Publications

    Educational Materials (11)

    hPSC Differentiation: Tools for Pluripotent Stem Cell-Derived Research
    Brochure
    hPSC Differentiation: Tools for Pluripotent Stem Cell-Derived Research
    STEMdiff™ Pancreatic Progenitor Kit
    Brochure
    STEMdiff™ Pancreatic Progenitor Kit
    Products for Human Pluripotent Stem Cells
    Brochure
    Products for Human Pluripotent Stem Cells
    qPCR Arrays for Cell Characterization
    Brochure
    qPCR Arrays for Cell Characterization
    How to Prepare Single Cells from PSC-Derived Epithelial Cultures for Flow Cytometry Analysis
    Protocol
    How to Prepare Single Cells from PSC-Derived Epithelial Cultures for Flow Cytometry Analysis
    SnapShot: GI Tract Development
    Wallchart
    SnapShot: GI Tract Development
    Directed Differentiation of Pluripotent Stem Cells
    Wallchart
    Directed Differentiation of Pluripotent Stem Cells
    STEMdiff™ Kits for Robust and Efficient Differentiation of hPSCs to Multiple Cell Types
    20:24
    Webinar
    STEMdiff™ Kits for Robust and Efficient Differentiation of hPSCs to Multiple Cell Types
    Modeling Human Gastrointestinal Development and Disease Using Pluripotent Stem Cells
    56:31
    Webinar
    Modeling Human Gastrointestinal Development and Disease Using Pluripotent Stem Cells
    Highly Efficient and Reproducible Differentiation of hPSCs to PDX1+ NKX6.1+ Pancreatic Progenitors
    Scientific Poster
    Highly Efficient and Reproducible Differentiation of hPSCs to PDX1+ NKX6.1+ Pancreatic Progenitors
    Reproducible and Efficient Differentiation of Human Pluripotent Stem Cells to Pancreatic Progenitors Using a Novel Serum-Free Medium
    Scientific Poster
    Reproducible and Efficient Differentiation of Human Pluripotent Stem Cells to Pancreatic Progenitors...

    We get a really great efficiency of differentiation in several pluripotent stem cell lines determined by expression of key markers: upregulation of PDX1, SOX9, FOXA2 and GATA4, and down-regulation of Sox17. We can generate these pancreatic progenitor cells reproducibly and efficiently; variability within the protocol is low.

    Drs. Jamie Trott and Ray Dunn, A*STAR Institute of Medical Biology (IMB), Singapore

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