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PneumaCult™ Airway Organoid Kit

Serum- and BPE-free medium for efficient establishment and differentiation of airway organoids
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产品优势


  • Three-dimensional in vitro system that recapitulates key characteristics of the in vivo human airway

  • Complete media formulation for expansion and differentiation of human airway epithelial cells, compatible with PneumaCult™-Ex Plus

  • Convenient format and easy-to-use protocol that delivers consistent results

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    概述
    实验数据
    产品说明书及文档
    应用领域
    相关材料与文献
    PneumaCult™ Airway Organoid Kit is a serum- and BPE-free culture medium system for efficient establishment and differentiation of airway organoids derived from human bronchial epithelial cells (HBECs).
    After 2D expansion in PneumaCult™-Ex Plus Medium (#05040), PneumaCult™ Airway Organoid Seeding Medium allows for initiation of 3D organoid culture, then PneumaCult™ Airway Organoid Differentiation Medium is used to obtain morphologically relevant airway organoids. Airway organoids provide a functional in vitro organotypic culture system for studying the airway epithelium. When fully differentiated, the organoids exhibit a centralized lumen surrounded by a polarized airway epithelial cell layer, comprised of differentiated cell types, such as ciliated cells and goblet cells. Applications of airway organoid cultures include modeling lung diseases, drug screening for efficacy and toxicity, and studying the functions of the airway epithelium.

    Browse our Frequently Asked Questions (FAQs) about culturing airway organoids culture using PneumaCult™.

    Should you intend to use this product for commercial purposes, please contact HUB at www.huborganoids.nl for a commercial use license or for clarifications in relation to HUB licensing.

    Data Figures

    Schematic of human airway organoid culture workflow using PneumaCult™ Airway Organoid Kit

    Figure 1. Overview of the PneumaCult™ Culture System for Human Airway Organoid Generation

    In the early two-dimensional expansion phase of the human airway organoid culture procedure, HBECs are expanded using PneumaCult™-Ex Plus Medium. The HBECs are then embedded into a Matrigel® dome and expanded for 4 - 7 days using PneumaCult™ Airway Organoid Seeding Medium. Following the expansion, the HBECs are differentiated using PneumaCult™ Airway Organoid Differentiation Medium for an additional 21+ days.

    Fully differentiated human airway organoids generated using PneumaCult™ Airway Organoid Kit display physiologically relevant morphology.

    Figure 2. Fully Differentiated Human Airway Organoids Generated Using PneumaCult™ Airway Organoid Kit

    (A) Bright-field image of airway organoids growing in PneumaCult™ Airway Organoid Seeding Medium at day 7 exhibit basal cell spheroid morphology. (B) Bright-field image of airway organoids differentiated in PneumaCult™ Airway Organoid Differentiation Medium at day 21 exhibit hollow lumens. (C) Airway organoid stained for ZO-1 (junction protein marker, red), MUC5AC (goblet cell marker, purple), AC-Tubulin (ciliated cell marker, green), and DAPI (nuclei, blue).

    Comparison of Forskolin-treated airway organoids derived from healthy donors and CF donors.

    Figure 3. Forskolin-Induced Swelling of Airway Organoids

    (A) Forskolin-treated organoids derived from healthy donors increased in size compared to the DMSO control, indicating functional CFTR protein expression. (B) Forskolin-induced swelling is lost in organoids derived from CF donors, but re-established in VX-809-treated airway organoids. Error bars represent ± 95% confidence interval for the mean (n=3). Bright-field images of airway organoids taken during the Forskolin swelling assay at (C) 0 hours and (D) 6 hours show organoid swelling after treatment.

    Ciliated cell percentage in fully differentiated airway organoids derived from healthy and cystic fibrosis (CF) donors using PneumaCult™ Airway Organoid Kit at different passages.

    Figure 4. Fully Differentiated Airway Organoids Retain Morphological Characteristics at Different Passages

    The ciliated cell percentage in organoids grown from (A) healthy and (B) CF donors using PneumaCult™ Airway Organoid Kit increased from P3 to P5. The total and ciliated cells were counted using a hemocytometer. Error bars represent ± 95% confidence interval for the mean (n=3).

    Protocols and Documentation

    Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

    Document Type
    Product Name
    Catalog #
    Lot #
    Language
    Document Type
    Product Information Sheet
    Product Name
    PneumaCult™ Airway Organoid Kit
    Catalog #
    05060
    Lot #
    All
    Language
    English
    Document Type
    Safety Data Sheet 1
    Product Name
    PneumaCult™ Airway Organoid Kit
    Catalog #
    05060
    Lot #
    All
    Language
    English
    Document Type
    Safety Data Sheet 2
    Product Name
    PneumaCult™ Airway Organoid Kit
    Catalog #
    05060
    Lot #
    All
    Language
    English
    Document Type
    Safety Data Sheet 3
    Product Name
    PneumaCult™ Airway Organoid Kit
    Catalog #
    05060
    Lot #
    All
    Language
    English

    Applications

    This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

    Research Area
    Workflow Stages
    Workflow Stages for Organoids
    View All

    Resources and Publications

    Educational Materials (22)

    Pulmonary Research
    Brochure
    Pulmonary Research
    Organoids
    Brochure
    Organoids
    Dissociation of Mouse Lung Tissue into a Single-Cell Suspension
    Protocol
    Dissociation of Mouse Lung Tissue into a Single-Cell Suspension
    Forskolin-Induced Swelling of Airway Organoids Derived from Healthy and Cystic Fibrosis Donors
    Protocol
    Forskolin-Induced Swelling of Airway Organoids Derived from Healthy and Cystic Fibrosis Donors
    How to Process Epithelial Organoids and Organoid-Derived Epithelial Monolayers for RNA Isolation
    Protocol
    How to Process Epithelial Organoids and Organoid-Derived Epithelial Monolayers for RNA Isolation
    Performing Immunocytochemical Staining of Epithelial Organoids
    Protocol
    Performing Immunocytochemical Staining of Epithelial Organoids
    Cellular Organization and Biology of the Respiratory System
    Wallchart
    Cellular Organization and Biology of the Respiratory System
    Dynamic Modeling in Organoids
    Wallchart
    Dynamic Modeling in Organoids
    What Are the Impacts of New Legislative Actions on Drug Development?
    3:07
    Video
    What Are the Impacts of New Legislative Actions on Drug Development?
    Beating Cilia of Airway Organoids Grown in PneumaCult™
    0:30
    Video
    Beating Cilia of Airway Organoids Grown in PneumaCult™
    In Vitro Human Airway Modeling Using Primary Airway Epithelial Cells
    18:41
    Webinar
    In Vitro Human Airway Modeling Using Primary Airway Epithelial Cells
    Optimization of Normal Human Bronchial Epithelial Cell 3D Cultures for In Vitro Lung Model Studies
    12:13
    Webinar
    Optimization of Normal Human Bronchial Epithelial Cell 3D Cultures for In Vitro Lung Model Studies
    Organoid-Based Assays for Disease Modeling
    24:55
    Webinar
    Organoid-Based Assays for Disease Modeling
    Reliable In Vitro Culture Solutions for Your Alveolar Research
    18:52
    Webinar
    Reliable In Vitro Culture Solutions for Your Alveolar Research
    Studying Respiratory Viruses with ALI Cultures
    6:56
    Webinar
    Studying Respiratory Viruses with ALI Cultures
    Elucidating Parechovirus A Pathogenesis Using Organoid Models
    30:09
    Webinar
    Elucidating Parechovirus A Pathogenesis Using Organoid Models
    Using Stem Cell-Based Models in Drug Discovery
    46:49
    Webinar
    Using Stem Cell-Based Models in Drug Discovery
    HUB & STEMCELL Organoids as Models of Infectious Disease Mini-Symposium
    1:10:09
    Webinar
    HUB & STEMCELL Organoids as Models of Infectious Disease Mini-Symposium
    Human Airway Organoids to Study Host-Pathogen Interactions
    35:24
    Webinar
    Human Airway Organoids to Study Host-Pathogen Interactions
    Studying Cystic Fibrosis Using Primary Human Nasal Epithelial Cells
    51:30
    Webinar
    Studying Cystic Fibrosis Using Primary Human Nasal Epithelial Cells
    Human Lung Development and Disease: Lessons from Organoids
    26:16
    Webinar
    Human Lung Development and Disease: Lessons from Organoids
    Dr. Bas Trietsch
    Interview
    Dr. Bas Trietsch
    Legal Statement: This product was developed under a license to intellectual property owned by Hubrecht Organoid Technology (HUB). This product is sold for research use only. Purchase of this product does not include the right to use it for drug screening aiming for commercial gain, equipment validation, biobanking, or for other commercial purposes. Purchasers wishing to use the product for purposes other than basic research use should contact HUB at www.huborganoids.nl to obtain a further license. Purchasers may apply for a License from HUB, which will not be unreasonably withheld by HUB. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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