mTeSR™3D

Serum-free media for suspension culture of human ES and iPS cells

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mTeSR™3D was developed for expansion and scale-up of undifferentiated human embryonic stem (ES) cells and human induced pluripotent stem (iPS) cells as aggregates in 3D suspension culture. The novel fed-batch workflow saves time and media. Daily feeds replenish nutrients and eliminate the need for medium exchanges on non-passaging days.

mTeSR™3D is a specialised and serum-free formulation. This culture system is compatible with a range of suspension culture vessels.

Data Figures

Figure 1. hPSC growth in mTeSR™3D

mTeSR™3D supports expansion of hES and hiPS cells over multiple passages in aggregate suspension culture. Shown are cumulative viable cell numbers for (A) hESC line H9 cultured in shaker bottles (10 passages) and (B) hiPSC line STiPS-F016 cultured in spinner flasks (5 passages). Some cell lines may experience 1 passage of adaptation from 2D to 3D, as shown in (B).

Figure 2. hPSC aggregate morphology in mTeSR3D

Representative images of hPSC aggregates cultured in mTeSR™3D. (A) H9 cells (B) H7 cells (C) STiPS-F016 cells.


Figure 3. Expression of undifferentiated cell markers

hPSCs cultured in mTeSR™3D maintain high expression of OCT4. (A) H9 cells after 10 passages in shaker bottles. (B) STiPS-F016 cells after 5 passages in spinner flasks. Representative flow cytometry plots shown.

Figure 4. Tri-lineage Differentiation

hPSCs cultured in mTeSR™3D can be efficiently differentiated to all three germ layers. Trilineage differentiation potential was assessed using the STEMdiff™ Trilineage Differentiation Kit (Cat # 05230).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

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Product Name
mTeSR™3D
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03950
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All
Language
English
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Technical Manual
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mTeSR™3D
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03950
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All
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English
Document Type
Safety Data Sheet 1
Product Name
mTeSR™3D
Catalog #
03950
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All
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English
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Safety Data Sheet 2
Product Name
mTeSR™3D
Catalog #
03950
Lot #
All
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English
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Safety Data Sheet 3
Product Name
mTeSR™3D
Catalog #
03950
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All
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English
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Safety Data Sheet 4
Product Name
mTeSR™3D
Catalog #
03950
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Legal Statement: mTeSR™3D is manufactured and sold under global exclusive license from Accellta for culture medium for hPSCs in suspension under feeder-free, non-adherent conditions. This product was developed under license to intellectual property owned by WiCell™ Research Institute. This product is sold for research use only (whether the buyer is an academic or for-profit entity) under a non-transferable, limited-use license. Purchase of this product does not include the right to sell, use or otherwise transfer this product for commercial purposes (i.e., any activity undertaken for consideration, such as use of this product for manufacturing, or resale of this product or any materials made using this product, or use of this product or any materials made using this product to provide services) or clinical use (i.e., administration of this product or any material using this product to humans) or the right to implant any material made using this product into an animal by, or in collaboration with, a for-profit entity, for purposes other than basic pre-clinical research applications (including without limitation teratoma assays) to validate the function of the cells. Purchasers who do not agree to the terms and conditions set forth above should return the product in acceptable conditions to the seller for a refund. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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